Top 2 Biorxiv Papers Today in Plant Biology


2.033 Mikeys
#1. A modular cloning toolkit for genome editing in plants
Florian Hahn, Andrey Korolev, Laura Sanjurjo Loures, Vladimir Nekrasov
The modular cloning (MoClo), based on the Golden Gate (GG) method, has enabled development of cloning systems with standardised genetic parts, e.g. promoters, coding sequences or terminators, that can be easily interchanged and assembled into expression units, which in their own turn can be further assembled into higher order multigene constructs. Here we present an expanded cloning toolkit that contains modules encoding a variety of CRISPR/Cas-based nucleases and their corresponding guide RNA backbones. Among other components, the toolkit includes a number of promoters that allow expression of CRISPR/Cas nucleases (or any other coding sequences) and their guide RNAs in monocots and dicots. As part of the toolkit, we present a set of modules that enable quick and facile assembly of tRNA-sgRNA polycistronic units without a PCR step involved. We believe the toolkit will contribute towards wider adoption of the CRISPR/Cas genome editing technology and modular cloning by researchers across the plant science community.
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PromPreprint: A modular cloning toolkit for genome editing in plants https://t.co/657npT1cV6
plantae_org: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
phdilly: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
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SelvarajGuru: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
SueRhee2: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
TJesse62: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
drk0311: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
Luke_Nikolov: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
ClemMarchal: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
laplap10: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
alvaroffer: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
Georgeudea: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
rene_inckemann: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
Kylelauersen: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
Kachroo_Lab: RT @PromPreprint: A modular cloning toolkit for genome editing in plants https://t.co/657npT1cV6
summerbeginner: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
SorenSeifi: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
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Debatosh2016: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
chinnen1982: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
ramki17071993: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
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Alex_Kiryushkin: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
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MiguelBotella4: RT @biorxiv_plants: A modular cloning toolkit for genome editing in plants https://t.co/Qz7RCpttHb #biorxiv_plants
DuongDoanMiche1: RT @biorxivpreprint: A modular cloning toolkit for genome editing in plants https://t.co/DZ1rIjc1BH #bioRxiv
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2.002 Mikeys
#2. Characterizing allele-by-environment interactions using maize introgression lines
Zhi Li, Sara B Tirado, Dnyaneshwar C Kadam, Lisa Coffey, Nathan D Miller, Edgar P Spalding, Aaron J Lorenz, Natalia de Leon, Shawn M Kaeppler, Patrick S Schnable, Nathan M Springer, Candice N Hirsch
Relatively small genomic introgressions containing quantitative trait loci can have significant impacts on the phenotype of an individual plant. However, the magnitude of phenotypic effects for the same introgression can vary quite substantially in different environments due to allele-by-environment interactions. To study potential patterns of allele-by-environment interactions, fifteen near-isogenic lines (NILs) with >90% B73 genetic background and multiple Mo17 introgressions were grown in 16 different environments. These environments included five geographical locations with multiple planting dates and multiple planting densities. The phenotypic impact of the introgressions was evaluated for up to 26 traits that span different growth stages in each environment to assess allele-by-environment interactions. Results from this study showed that small portions of the genome can drive significant genotype-by-environment interaction across a wide range of vegetative and reproductive traits, and the magnitude of the...
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TrickerPenny: RT @biorxiv_plants: Characterizing allele-by-environment interactions using maize introgression lines https://t.co/2aeK0FnMiw #biorxiv_pla…
zhenbin_hu: RT @biorxiv_plants: Characterizing allele-by-environment interactions using maize introgression lines https://t.co/2aeK0FnMiw #biorxiv_pla…
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