Top 2 Biorxiv Papers Today in Cell Biology


1.997 Mikeys
#1. Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes
Pooja Suresh, Alexandra F Long, Sophie Dumont
The spindle generates force to segregate chromosomes at cell division. In mammalian cells, kinetochore-fibers connect chromosomes to the spindle. The dynamic spindle anchors kinetochore-fibers in space and time to coordinate chromosome movement. Yet, how it does so remains poorly understood as we lack tools to directly challenge this anchorage. Here, we adapt microneedle manipulation to exert local forces on the spindle with spatiotemporal control. Pulling on kinetochore-fibers reveals that the spindle retains local architecture in its center on the seconds timescale. Upon pulling, sister, but not neighbor, kinetochore-fibers remain tightly coupled, restricting chromosome stretching. Further, pulled kinetochore-fibers freely pivot around poles but not around chromosomes, retaining their orientation within 3 μm of chromosomes. This local reinforcement has a 20 s lifetime, and requires the microtubule crosslinker PRC1. Together, these observations indicate short-lived, specialized reinforcement of the kinetochore-fiber in the...
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biorxivpreprint: Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes https://t.co/OceIui00cY #bioRxiv
biorxiv_cellbio: Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes https://t.co/1hYyqVNWFm #biorxiv_cellbio
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1.996 Mikeys
#2. PERICYTE-LIKE CELLS UNDERGO TRANSCRIPTIONAL REPROGRAMMING AND DISTINCT FUNCTIONAL ADAPTATIONS IN ACUTE LUNG INJURY
Chi F Hung, Sarah Holton, Yu-Hua Chow, W. Conrad Liles, Sina Gharib, William A Altemeier
Background: We previously reported on the role of pericyte-like cells as functional sentinel immune cells in lung injury. However, much about the biological role of pericytes in lung injury remains unknown. Lung pericyte-like cells are well-positioned to sense disruption to the epithelial barrier and coordinate local inflammatory responses due to their anatomic niche within the alveoli. In this report, we characterized transcriptional responses and functional changes in pericyte-like cells following activation by alveolar components from injured and uninjured lungs in a mouse model of acute lung injury (ALI). Methods: We purified pericyte-like cells from lung digests using PDGFRβ as a selection marker and expanded them in culture as previously described (1). We induced sterile acute lung injury in mice with recombinant human Fas ligand (rhFasL) instillation followed by mechanical ventilation (1). We then collected bronchoalveolar lavage fluid (BALF) from injured and uninjured mice. Purified pericyte-like cells in culture were...
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