Top 9 Biorxiv Papers Today in Cell Biology


2.082 Mikeys
#1. A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division
Lovorka Stojic, Aaron TL Lun, Patrice Mascalchi, Christina Ernst, Aisling M Redmond, Jasmin Mangei, Alexis R Barr, Vicky Bousgouni, Chris Bakal, John C Marioni, Duncan T Odom, Fanni Gergely
Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. The role of long noncoding RNAs (lncRNAs) in controlling these processes however remains largely unexplored. To identify lncRNAs with mitotic functions, we performed a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs. By investigating major hallmarks of cell division such as chromosome segregation, mitotic duration and cytokinesis, we discovered numerous lncRNAs with functions in each of these processes. The chromatin-associated lncRNA, linc00899, was selected for in-depth studies due to the robust mitotic delay observed upon its depletion. Transcriptome analysis of linc00899-depleted cells together with gain-of-function and rescue experiments across multiple cell types identified the neuronal microtubule-binding protein, TPPP/p25, as a target of linc00899. Linc00899 binds the genomic locus of TPPP/p25 and suppresses its transcription through...
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IgorUlitsky: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
Alexis_Lomakin: RT @biorxiv_cellbio: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/sPHB3EhtNo #b…
grnfluoresceblg: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
BorisLenhard: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
ShorterLabGroup: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
PrecursorCell: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
Chromatin_: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
YangWeibing: RT @biorxiv_cellbio: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/sPHB3EhtNo #b…
AGuleren: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
vikasyadav9189: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
assaf98973488: RT @biorxivpreprint: A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division https://t.co/MLytc5DhAk #b…
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Authors: 12
Total Words: 0
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2.077 Mikeys
#2. NBR1-mediated p62-liquid droplets enhance the Keap1-Nrf2 system
Masaaki Komatsu, Pablo Sánchez-Martín, Shun Kageyama, Yu-Shin Sou
p62/SQSTM1 is a multivalent protein that has an ability to cause a liquid-liquid phase separation and serves as a receptor protein that participates in cargo isolation during selective autophagy. This protein is also involved in the non-canonical activation of the Keap1-Nrf2 system, a major oxidative stress response pathway. Here we show a role of Neighbor of BRCA1 gene 1 (NBR1), an autophagy receptor structurally similar to p62/SQSTM1, in the p62-liquid droplet formation and the Keap1-Nrf2 pathway. The overexpression of NBR1 blocked selective degradation of p62/SQSTM1 through autophagy and promoted the accumulation and phosphorylation of p62/SQSTM1 in liquid-like bodies, which is required for the activation of Nrf2. NBR1 was induced in response to oxidative stress, and then the p62-mediated Nrf2 activation was up-regulated. Conversely, loss of Nbr1 suppresses not only the formation of p62/SQSTM1-liquid droplets but also p62-dependent Nrf2 activation during oxidative stress. Taken together, our results show that NBR1 mediates...
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biorxivpreprint: NBR1-mediated p62-liquid droplets enhance the Keap1-Nrf2 system https://t.co/unNmhyolt8 #bioRxiv
biorxiv_cellbio: NBR1-mediated p62-liquid droplets enhance the Keap1-Nrf2 system https://t.co/lZWb1RCNZ7 #biorxiv_cellbio
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Authors: 4
Total Words: 0
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1.999 Mikeys
#3. Visualisation of ribosomes in Drosophila axons using Ribo-BiFC
Anand Kumar Singh, Akilu Abdullahi, Matthias Soller, Alexandre David, Saverio Brogna
Rates of protein synthesis and the number of translating ribosomes vary greatly between different cells in various cell states. The distribution of assembled, and potentially translating, ribosomes within cells can be visualised in Drosophila by using Biomolecular Fluorescence Complementation (BiFC) to monitor the interaction between tagged pairs of 40S and 60S ribosomal proteins (RPs) that are close neighbours across inter-subunit junctions in the assembled 80S ribosome. Here we describe UAS-regulated transgenes that express two novel RP pairs tagged with Venus-based BiFC fragments that considerably increase the sensitivity of this technique that we termed Ribo-BiFC, and should provide a convenient way of monitoring the local distribution of ribosomes in most Drosophila cells. This improved method clearly visualises 80S ribosomes in larval photoreceptors and in other neurons. Assembled ribosomes are most abundant in the various neuronal cell bodies, but they are also present along the lengths of axons and are concentrated in...
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biorxivpreprint: Visualisation of ribosomes in Drosophila axons using Ribo-BiFC https://t.co/aCyNURWwvj #bioRxiv
biorxiv_cellbio: Visualisation of ribosomes in Drosophila axons using Ribo-BiFC https://t.co/EYYpFcnOyw #biorxiv_cellbio
josephwgordon: RT @biorxiv_cellbio: Visualisation of ribosomes in Drosophila axons using Ribo-BiFC https://t.co/EYYpFcnOyw #biorxiv_cellbio
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Authors: 5
Total Words: 5911
Unqiue Words: 1749

1.998 Mikeys
#4. Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography
Marie-Christin Spindler, Sebastian Filbeck, Christian Stigloher, Ricardo Benavente
The synaptonemal complex is a multiprotein complex, which mediates the synapsis and recombination between homologous chromosomes during meiosis. The complex is comprised of two lateral elements and a central element connected by perpendicular transverse filaments (TFs). A 3D model based on actual morphological data of the SC is missing. Here, we applied electron tomography (ET) and manual feature extraction to generate a quantitative 3D model of the murine SC. We quantified the length (90 nm) and width (2 nm) of the TFs. Interestingly, the 80 TFs/μm are distributed asymmetrically in the central region of the SC challenging available models of SC organization. Furthermore, our detailed 3D topological analysis does not support a bilayered organization of the central region as proposed earlier. Overall, our quantitative analysis is relevant to understand the functions and dynamics of the SC and provides the basis for analyzing multiprotein complexes in their morphological context using ET.
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biorxivpreprint: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography https://t.co/QZlczNEA2F #bioRxiv
biorxiv_cellbio: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography https://t.co/aoWyY9QFCW #biorxiv_cellbio
meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
NeedhiBhalla: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
NeedhiBhalla: RT @biorxiv_cellbio: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography https://t.co/aoWyY9QFCW #biorxiv_ce…
cohen_lab: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
lichtenmj: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
biobosie: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
BhallaLab: RT @biorxiv_cellbio: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography https://t.co/aoWyY9QFCW #biorxiv_ce…
ChristineMezard: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
MonicaPradillo: RT @meiosis_papers: Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography | Benavente R https://t.co/l9T0k5OsuC
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Authors: 4
Total Words: 8222
Unqiue Words: 2274

1.998 Mikeys
#5. Heparanase-2 protects from endothelial injury by inhibiting TLR4 signaling
Yulia Kiyan, Sergey Tkachuk, Kestutis Kurselis, Nelli Shushakova, Klaus Stahl, Damilola Dawodu, Roman Kiyan, Boris N Chichkov, Hermann Haller
Objective: The endothelial glycocalyx and the regulation of its shedding are important to vascular health. Endo-β-D-glucuronidase heparanase-1 (HPSE1) is the only enzyme that can shed heparan sulfate. However, the mechanisms are not well understood. Approach and results: To investigate HPSE1 and its endogenous inhibitor, heparanase-2 (HPSE2), we used cell culture, lentiviral protein overexpression, a microfluidic chip model of cell culture under shear stress conditions, and lipopolysaccharide (LPS) injections in mice. We show that HPSE1 activity aggravated Toll-like receptor 4 (TLR4)-mediated response of endothelial cells to LPS. On the contrary, HPSE2 overexpression was protective. The microfluidic chip flow model confirmed that HPSE2 prevented heparan sulfate shedding by HPSE1. Furthermore, heparan sulfate did not interfere with cluster of differentiation-14 (CD14)-dependent LPS binding, but instead reduced the presentation of the LPS-CD14 complex to TLR4. HPSE2 reduced LPS-mediated TLR4 activation by LPS, subsequent cell...
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Authors: 9
Total Words: 0
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1.996 Mikeys
#6. Kinase inhibition of G2019S-LRRK2 restores autolysosome formation and function to reduce endogenous alpha-synuclein intracellular inclusions
Julia Obergasteiger, Giulia Frapporti, Giulia Lamonaca, Sara Pizzi, Anne Picard, Franscesca Pischedda, Giovanni Piccoli, Evy Lobbestael, Veerle Baekelandt, Andrew A Hicks, Corrado Corti, Peter P Pramstaller, Mattia Volta
The Parkinson's disease (PD)-associated kinase Leucine-Rich Repeat Kinase 2 (LRRK2) is a potent modulator of autophagy and impacts on lysosome biology and function, but unclarity exists on the precise mechanics of its role and the direction of this modulation. LRRK2 is also involved in the degradation of pathological alpha-synuclein, with pathogenic mutations precipitating neuropathology in cellular and animal models of PD, and most LRRK2 familial cases manifesting with Lewy neuropathology. Defects in autophagic processing and lysosomal degradation of alpha-synuclein have been postulated to underlie its accumulation and onset of neuropathology. Thus, it is critical to reconcile these independent pieces of information to obtain a comprehensive knowledge on LRRK2-associated pathology that could also be generalized to the idiopathic disease. Here, we report a focused investigation on the role of PD-causing G2019S-LRRK2 in the autophagy-lysosome pathway in a recombinant cell line model. Initially, we evaluated the effect of LRRK2...
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Authors: 13
Total Words: 9813
Unqiue Words: 2724

1.993 Mikeys
#7. CLEC-2 suppresses calcification in cultured osteoblasts
Takenori Kanai, Yoshihiko Sawa, Kenyo Takara, Koichiro Kajiwara, Takahiro Fujita, Naruhiko Sawa, Junro Yamashita, Yoshiaki Sato
Podoplanin is the only counter-receptor of platelet CLEC-2 and is expressing on mature osteoblast, but there is no report on the role of podoplanin and CLEC-2 in calcification. This study aimed to investigate the role of podoplanin binding to CLEC-2 in the calcification of osteoblasts carrying homozygously deleted Pdpn alleles (PdpnΔ/Δ) by heterozygously expressing collagen type I alpha 1 promoter (Col1a)-driven Cre recombinase. There were no macroscopic abnormalities in the bone and dentin of Col1a11-Cre;PdpnΔ/Δ mice but the coccygeal bone medullary cavity was very narrow. In the quantitative analysis for alizarin red-stained products and alkaline phosphatase activities on the cultured calvarial osteoblasts, the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts of both Pdpnfl/fl and Col1a11-Cre;PdpnΔ/Δ mice were significantly higher in the calcification medium than in the α-mem. Both the amounts of calcified products and alkaline phosphatase activity of calvarial osteoblasts from Pdpnfl/fl...
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Authors: 8
Total Words: 7804
Unqiue Words: 2378

1.993 Mikeys
#8. A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag
Inbar Segal, Dikla Nachmias, Eyal Arbely, Natalie Elia
In the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used live cell applications, partly because it needs to be adjusted to the specific protein under study. Here, we present a generic, 14-residues long, N-terminal tag for GCE-based labeling of proteins in live mammalian cells. Using this tag, we generated a library of GCE-based organelle markers, demonstrating the applicability of the tag for labeling a plethora of proteins and organelles. Finally, we show that the HA epitope, used as a backbone in our tag, can be substituted with other epitopes and, in some cases, can be completely removed, reducing the tag length to 5 residues. The GCE-tag presented here offers a powerful, easy-to-implement tool for live cell labeling of cellular proteins with small and bright probes.
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Authors: 4
Total Words: 7226
Unqiue Words: 2330

1.989 Mikeys
#9. Functional reconstitution of TatB into thylakoidal Tat translocase
Sarah Zinecker, Mario Jakob, Ralf Bernd Klösgen
We have established an experimental system for the functional analysis of thylakoidal TatB, a component of the membrane-integral TatBC receptor complex of the thylakoidal Twin-arginine protein transport (Tat1) machinery. For this purpose, the intrinsic TatB activity of isolated pea thylakoids was inhibited by affinity-purified antibodies and substituted by supplementing the assays with TatB protein either obtained by in vitro translation or purified after heterologous expression in E. coli. Tat transport activity of such reconstituted thylakoids, which was analyzed with the authentic Tat substrate pOEC16, reached routinely 20 - 25% of the activity of mock-treated thylakoid vesicles analysed in parallel. In contrast, supplementation of the assays with the purified antigen comprising all but the N-terminal transmembrane helix of thylakoidal TatB did not result in Tat transport reconstitution which confirms that transport relies strictly on the activity of the TatB protein added and is not due to restoration of the intrinsic TatB...
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Authors: 3
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