Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation
Although female pluripotency significantly differs to male, complications with in vitro culture of female embryonic stem cells (ESC) have severely limited the use and study of these cells. We report a replenishable female ESC system, Xmas, that has enabled us to optimise a protocol for preserving the XX karyotype. Our protocol also improves male ESC fitness. We utilised our Xmas ESC system to screen for regulators of the female-specific process of X chromosome inactivation, revealing chromatin remodellers Smarcc1 and Smarca4 as key regulators of establishment of X inactivation. The remodellers create a nucleosome depleted region at gene promotors on the inactive X during exit from pluripotency, without which gene silencing fails. Our female ESC system provides a tractable model for XX ESC culture that will expedite study of female pluripotency and has enabled us to discover new features of the female-specific process of X inactivation.
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Andrew Keniry (add twitter)
Natasha Jansz (edit)
Linden J. Gearing (add twitter)
Iromi Wanigasuriya (edit)
Joseph Chen (edit)
Christian M Nefzger (edit)
Peter F Hickey (edit)
Quentin Gouil (edit)
Joy Liu (edit)
Kelsey Breslin (add twitter)
Megan Iminitoff (add twitter)
Tamara Beck (add twitter)
Andres Tapia del Fierro (add twitter)
Sarah A Kinkel (add twitter)
Phillippa C Taberlay (edit)
Tracy Willson (edit)
Miha Pakusch (add twitter)
Matthew E Ritchie (add twitter)
Douglas J Hilton (add twitter)
Jose Maria Polo (add twitter)
Marnie E Blewitt (add twitter)
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Cell Biology

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09/13/19 03:01PM
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PromPreprint: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation https://t.co/tA6IYOz00S
biorxiv_cellbio: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X ... https://t.co/Vi03mCL0F2 #biorxiv_cellbio
biorxivpreprint: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation https://t.co/YfBLXx5vjT #bioRxiv
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