Blastodermal cuticle formation contributes to desiccation resistance in springtail eggs: eco-evolutionary implications for insect terrestrialization
Land colonization was a major event in the history of life. Among animals, insects had a staggering terrestrialization success, due to traits usually associated with post embryonic life stages, while the egg stage has been largely overlooked in comparative studies. In many insects, after blastoderm differentiation, the extraembryonic serosal tissue wraps the embryo and synthesizes the serosal cuticle, an extracellular matrix that lies beneath the eggshell and protects the egg against water loss. In contrast, in non-insect hexapods such as springtails (Collembola) the early blastodermal cells synthesize a blastodermal cuticle. Here, we investigate the relationship between blastodermal cuticle formation and egg resistance to desiccation in the springtails Orchesella cincta and Folsomia candida, two species with different oviposition tenvironments and developmental rates. The blastodermal cuticle becomes externally visible in O. cincta and F. candida at 22 and 29% of embryogenesis, respectively. To contextualize, we describe the stages of springtail embryogenesis, exemplified by F. candida. Physiological assays showed that blastodermal cuticle formation supports egg viability in a dry environment, significantly contributing to hatching success. However, protection differs between species: while O. cincta eggs survive at least 2 hours outside a humid environment, the survival period recorded for F. candida eggs is only 15 minutes, which correlates with this species' requirement for humid microhabitats. We suggest that the formation of this protective cuticle is an ancestral trait among hexapods, predating and facilitating the process of terrestrialization that occurred during insect evolution.
NurtureToken New!

Token crowdsale for this paper ends in

Buy Nurture Tokens

Authors

Are you an author of this paper? Check the Twitter handle we have for you is correct.

Helena Carolina Martins Vargas (add twitter)
Kristen A Panfilio (add twitter)
Dick Roelofs (edit)
Gustavo Lazzaro Rezende (add twitter)
Category

Zoology

Ask The Authors

Ask the authors of this paper a question or leave a comment.

Read it. Rate it.
#1. Which part of the paper did you read?

#2. The paper contains new data or analyses that is openly accessible?
#3. The conclusion is supported by the data and analyses?
#4. The conclusion is of scientific interest?
#5. The result is likely to lead to future research?

Github
User:
None (add)
Repo:
None (add)
Stargazers:
0
Forks:
0
Open Issues:
0
Network:
0
Subscribers:
0
Language:
None
Youtube
Link:
None (add)
Views:
0
Likes:
0
Dislikes:
0
Favorites:
0
Comments:
0
Other
Sample Sizes (N=):
Inserted:
Words Total:
Words Unique:
Source:
Abstract:
None
09/12/19 05:03PM
9,610
3,010
Tweets
biorxivpreprint: Blastodermal cuticle formation contributes to desiccation resistance in springtail eggs: eco-evolutionary implications for insect terrestrialization https://t.co/lU8dOSpl5W #bioRxiv
Images
Related