XPRESSyourself: Enhancing and Automating the Ribosome Profiling and RNA-Seq Analysis Toolkit
Nucleic acid sequencing is a powerful research tool that has become routine; however, computational bottlenecks hinder many users. XPRESSyourself is a ribosome profiling and RNA-Seq analytical pipeline that aims to eliminate these barriers, standardize in silico protocols, and decrease time-to-discovery. XPRESSyourself additionally introduces tools missing from current ribosome profiling and RNA-Seq toolkits. Using XPRESSyourself to process publicly available ribosome profiling data, we were able to rapidly identify hypothetical mechanisms related to neurodegenerative phenotypes and neuroprotective mechanisms of the small-molecule ISRIB during acute cellular stress, highlighting the ability of XPRESSyourself to rapidly uncover novel biological insight from high-throughput sequence data.