Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag
Labeling and tracking biomolecules with fluorescent probes on the single molecule level enables quantitative insights into their dynamics in living cells. We previously developed Riboglow, a platform to label RNAs in live mammalian cells, consisting of a short RNA tag and a small organic probe that increases fluorescence upon binding RNA. Here, we demonstrate that Riboglow is capable of detecting and tracking single RNA molecules. We benchmark RNA tracking by comparing results with the established MS2 RNA tagging system. To demonstrate versatility of Riboglow, we assay translation on the single molecule level, where the translated mRNA is tagged with Riboglow and the nascent polypeptide is labeled with a fluorescent antibody. The growing effort to investigate RNA biology on the single molecule level requires sophisticated and diverse fluorescent probes for multiplexed, multi-color labeling of biomolecules of interest, and we present Riboglow as a new member in this toolbox.
NurtureToken New!

Token crowdsale for this paper ends in

Buy Nurture Tokens

Authors

Are you an author of this paper? Check the Twitter handle we have for you is correct.

ESTHER BRASELMANN (edit)
Timothy J Stasevich (edit)
Kenneth Lyon (add twitter)
Robert T Batey (add twitter)
AMY E PALMER (add twitter)
Category

Cell Biology

Ask The Authors

Ask the authors of this paper a question or leave a comment.

Read it. Rate it.
#1. Which part of the paper did you read?

#2. The paper contains new data or analyses that is openly accessible?
#3. The conclusion is supported by the data and analyses?
#4. The conclusion is of scientific interest?
#5. The result is likely to lead to future research?

Github
User:
None (add)
Repo:
None (add)
Stargazers:
0
Forks:
0
Open Issues:
0
Network:
0
Subscribers:
0
Language:
None
Youtube
Link:
None (add)
Views:
0
Likes:
0
Dislikes:
0
Favorites:
0
Comments:
0
Other
Sample Sizes (N=):
Inserted:
Words Total:
Words Unique:
Source:
Abstract:
None
07/13/19 02:02PM
7,996
2,293
Tweets
hauryliuk: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
Juli_Bla: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
petry594: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
DoubleAsh3: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
Parikki: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
poppingjun: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
AGuleren: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
TJesse62: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
HandeKarahan_: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
lacks22: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
VWosika: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
YangWeibing: RT @biorxiv_cellbio: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/MyTzJRzHwd #b…
Images
Related