An hourglass pattern of inter-embryo gene expression variability and of histone regulation in fly embryogenesis
Gene expression is in part a stochastic process, which is at odds with the need for precise expression levels in biological processes such as embryonic development. To study the tension between precision and stochasticity of gene expression during development, we quantified expression variability of single embryo transcriptomes throughout fly Drosophila melanogaster embryogenesis, from 150 samples over eight developmental stages. We found that expression variability follows an hourglass pattern, with lower variability at extended germband, the phylotypic stage. We explain this pattern by stronger histone modification mediated transcriptional noise control at this stage. In addition, we propose that histone modifications contribute to mutational robustness in regulatory elements, and thus to conserved expression levels. These results provide insight into the role of robustness in the phenotypic and genetic patterns of evolutionary conservation in animal development.
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Jialin Liu (edit)
Michael Frochaux (add twitter)
Vincent Gardeux (edit)
Bart Deplancke (edit)
Marc Robinson-Rechavi (edit)
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07/13/19 02:02PM
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biorxivpreprint: An hourglass pattern of inter-embryo gene expression variability and of histone regulation in fly embryogenesis https://t.co/QJkwslTbn2 #bioRxiv
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