A Scalable, Multiplexed Assay for Decoding Receptor-Ligand Interactions
Chemicals such as drugs, hormones, and odorants can have many potential interactions with endogenous targets, and uncovering these relationships is critical for understanding and modulating function. Mammalian olfactory receptors (ORs), a large family of G protein-coupled receptors, mediate olfaction through activation by small molecules. Each OR can respond to many odorants, and vice versa, making exploring this space one interaction at a time difficult. We developed a high-throughput receptor screening platform in human cell lines to screen libraries of chemicals against a multiplexed library of receptors using next-generation sequencing of barcoded genetic reporters. We screened three concentrations of 181 odorants, where in each well we record the activity of 39 ORs simultaneously, and identified 79 novel associations, including ligands for 15 orphan receptors. This platform allows the cost-effective mapping of large chemical libraries to receptor repertoires at scale.
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Eric M Jones (add twitter)
Rishi Jajoo (edit)
Daniel Cancilla (add twitter)
Nathan B Lubock (add twitter)
Jeff Wang (add twitter)
Megan Satyadi (add twitter)
Rocky Cheung (edit)
Claire de March (add twitter)
Hiroaki Matsunami (add twitter)
Sriram Kosuri (add twitter)
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Biochemistry

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06/28/18 11:47PM
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Tweets
LindowMorten: Nice use of RNASeq to assay multiple GPCRs in same well. HTP https://t.co/T7U4J7glkY
JerzySzablowski: Drug discovery in higher throughput. A Scalable, Multiplexed Assay for Decoding Receptor-Ligand Interactions https://t.co/C3lFxqrDB8
PromPreprint: A Scalable, Multiplexed Assay for Decoding Receptor-Ligand Interactions https://t.co/P8GplC5Jj1
rnomics: Top #tweeted story in #bioscience: A Scalable, Multiplexed Assay for Decoding R… https://t.co/P53yydtI6y, see more https://t.co/kME3G1o122
srikosuri: Really excited to share this new work from @emjbio, Rishi Jajoo, and others from the lab and Hiro Matsunami's lab @DukeU. We develop a system to deorphanize olfactory receptors using a new multiplexed GPCR screening platform... [1/5] https://t.co/dqFpq8GA8F
zandawala: RT @biorxivpreprint: A Scalable, Multiplexed Assay for Decoding Receptor-Ligand Interactions https://t.co/jXIsVMxbuK #bioRxiv
biorxivpreprint: A Scalable, Multiplexed Assay for Decoding Receptor-Ligand Interactions https://t.co/jXIsVMxbuK #bioRxiv
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