Top 1 Biorxiv Papers Today in Synthetic Biology


2.005 Mikeys
#1. Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes
Jaewan Jang, Sherin McDonald, Maruti Uppalapati, Andrew Woolley
Existing optogenetic tools for controlling protein-protein interactions are available in a limited number of wavelengths thereby limiting opportunities for multiplexing. The cyanobacteriochrome (CBCR) family of photoreceptors responds to an extraordinary range of colors, but light-dependent binding partners for CBCR domains are not currently known. We used a phage-display based approach to develop small (~50-residue) monomeric binders selective for the green absorbing state (Pg), or for the red absorbing state (Pr) of the CBCR Am1\_c0023g2 with a phycocyanobilin chromophore and also for the far-red absorbing state (Pfr) of Am1\_c0023g2 with a biliverdin chromophore. These bind in a 1:1 mole ratio with KDs for the target state from 0.2 to 2 μM and selectivities from 10 to 500-fold. We demonstrate green, orange, red, and far-red light-dependent control of protein-protein interactions in vitro and also in vivo where these multicolor optogenetic tools are used to control transcription in yeast.
more | pdf
Figures
Tweets
biorxivpreprint: Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes https://t.co/t59CPcvbYu #bioRxiv
OUPSynBio: We would welcome this submission: Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes #synbio #openaccess https://t.co/6hIN8JKoAj
sdelagrave: RT @biorxivpreprint: Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes https://t.co/t59CPcvbYu #bioRxiv
enerphyschem: RT @biorxivpreprint: Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes https://t.co/t59CPcvbYu #bioRxiv
tisrenk: RT @biorxivpreprint: Green, orange, red, and far-red optogenetic tools derived from cyanobacteriochromes https://t.co/t59CPcvbYu #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 4
Total Words: 7443
Unqiue Words: 2116

About

Assert is a website where the best academic papers on arXiv (computer science, math, physics), bioRxiv (biology), BITSS (reproducibility), EarthArXiv (earth science), engrXiv (engineering), LawArXiv (law), PsyArXiv (psychology), SocArXiv (social science), and SportRxiv (sport research) bubble to the top each day.

Papers are scored (in real-time) based on how verifiable they are (as determined by their Github repos) and how interesting they are (based on Twitter).

To see top papers, follow us on twitter @assertpub_ (arXiv), @assert_pub (bioRxiv), and @assertpub_dev (everything else).

To see beautiful figures extracted from papers, follow us on Instagram.

Tracking 189,566 papers.

Search
Sort results based on if they are interesting or reproducible.
Interesting
Reproducible
Feedback
Online
Stats
Tracking 189,566 papers.