Top 1 Biorxiv Papers Today in Synthetic Biology

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#1. Cell-free transcriptional regulation via nucleic-acid-based transcription factors
Leo Chou, William Shih
Cells execute complex transcriptional programs by deploying distinct protein regulatory assemblies that interact with cis-regulatory elements throughout the genome. Using concepts from DNA nanotechnology, we synthetically recapitulated this feature in cell-free gene networks actuated by T7 RNA polymerase (RNAP). Our approach involves engineering nucleic-acid hybridization interactions between a T7 RNAP site-specifically functionalized with single-stranded DNA (ssDNA), templates displaying cis-regulatory ssDNA domains, and auxiliary nucleic-acid assemblies acting as artificial transcription factors (TFs). By relying on nucleic-acid hybridization, de novo regulatory assemblies can be computationally designed to emulate features of protein-based TFs, such as cooperativity and combinatorial binding, while offering unique advantages such as programmability, chemical stability, and scalability. We illustrate the use of nucleic-acid TFs to implement transcriptional logic, cascading, feedback, and multiplexing. This framework will enable...
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leochou0814: Check out our latest work done at the @wyssinstitute using an oligo-modified T7 RNAP to direct transcription in cell-free systems! Now on bioarxiv:
pdmillett: Cell-free transcriptional regulation via nucleic-acid-based transcription factors
brian_tenner: RT @biorxivpreprint: Cell-free transcriptional regulation via nucleic-acid-based transcription factors #bioRxiv
Other stats
Sample Sizes : None.
Authors: 2
Total Words: 5096
Unqiue Words: 1688


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