Top 4 Biorxiv Papers Today in Plant Biology


2.015 Mikeys
#1. Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems
Deepanksha Arora, Nikolaj B. Abel, Chen Liu, Petra Van Damme, Lam Dai Vu, Anna Tornkvist, Francis Impens, Dominique Eeckhout, Alain Goossens, Geert De Jaeger, Thomas Ott, Panagiotis Moschou, Daniel Van Damme
The use of proximity-dependent biotin labelling (PDL) approaches coupled with mass spectrometry recently greatly advanced the identification of protein-protein interactions and study of protein complexation. PDL is based on the expression of a promiscuous biotin ligase (PBL), e.g. BirA* or a peroxidase fused to a bait protein of interest. In the presence of biotin as substrate, PBL enables covalent biotin labelling of proteins in the vicinity of the PBL-fused bait in vivo, allowing the subsequent capture and identification of interacting and neighbouring proteins without the need for the protein complex to remain intact during purification. To date, PDL has not been extensively used in plants. Here we present the results of a systematic multi-lab study applying a variety of PDL approaches in several plant systems under various conditions and bait proteins. We show that TurboID is the most promiscuous variant for PDL in plants and establish protocols for its efficient application. We demonstrate the applicability of TurboID in...
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maolopobo: Powerful and very interested to apply in moss! https://t.co/LC9VGVNW9V
Grandpa_Hiro: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
gangyu1987: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
xiaolin422: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
Adam_Mott: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
TomRegnault: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
JaviGallego80: RT @biorxiv_plants: Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems https://t.co/UFm9lrBruv…
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Authors: 13
Total Words: 14201
Unqiue Words: 4496

1.996 Mikeys
#2. Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
Bruno Pok Man Ngou, Hee-Kyung Ahn, Pingtao Ding, Amey Redkar, Hannah Brown, Yan Ma, Mark Youles, Laurence Tomlinson, Jonathan DG Jones
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effectors AvrRps4 and PopP2, are well studied, but how the RRS1/RPS4 complex activates early immediate downstream responses upon effector detection is still poorly understood. To study RRS1/RPS4 responses without the influence of cell-surface receptor immune pathways, we generated an Arabidopsis line with inducible expression of effector AvrRps4. Induction does not lead to hypersensitive cell death response (HR) but can induce electrolyte leakage, which often correlates with plant cell death. Activation of RRS1 and RPS4 without pathogens cannot activate mitogen-associated protein kinase cascades, but still activates upregulation of defense genes, and therefore resistance against bacteria.
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Authors: 9
Total Words: 9117
Unqiue Words: 3245

1.995 Mikeys
#3. Hemibiotrophic fungal pathogen induces systemic susceptibility and systemic shifts in wheat metabolome and microbiome composition
Heike Seybold, Tobias Demetrowitsch, M. Amine Hassani, Silke Szymczak, Ekaterina Reim, Janine Haueisen, Malte Ruehlemann, Andre Franke, Karin Schwarz, Eva H. Stukenbrock
Yield losses caused by fungal pathogens represent a major threat to global food production. One of the most devastating fungal wheat pathogens is Zymoseptoria tritici. Despite the importance of this fungus and wheat as main staple food crop the underlying mechanisms of plant-pathogen interactions are poorly understood. Here we present a conceptual framework based on coinfection assays, comparative metabolomics, and microbiome profiling to study the interaction of Z. tritici in susceptible and resistant wheat. We demonstrate that Z. tritici suppresses the production of immune-related metabolites in a susceptible cultivar. Remarkably, this fungus-induced immune suppression spreads within the leaf and even to other leaves, a previously undescribed phenomenon that we term "systemic induced susceptibility". Using a comparative metabolomics approach, we identified defense-related biosynthetic pathways that are suppressed and induced in susceptible and resistant cultivars, respectively. We show that these fungus-induced changes also...
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Authors: 10
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1.995 Mikeys
#4. Host defense responses of CO441 and CL30 maize lines to Fusarium graminearum analyzed by comparative label-free quantitative proteomics
Jason El Bilali, Lana Reid, Illimar Altosaar
Gibberella ear rot is a disease of maize associated with low yields and the production of harmful mycotoxins therein. The disease is caused by the infection of host Zea mays with fungal pathogen Fusarium graminearum. Resistant (CO441) and susceptible (CL30) inbred maize line kernels were inoculated with conidial suspensions of F. graminearum or water (controls). Ears of maize (cobs) from each line were harvested upon maturation and proteins were extracted from the embryo tissue of the kernels to study tissue-specific response of the host. Embryo proteins from both CO441 and CL30 lines were sequenced using mass spectrometry (LC-MS/MS) and quantified using Label Free Quantification (LFQ). Following filtering, 509 proteins were identified. These proteins were grouped into nine functional categories: Fusarium-derived, late embryogenesis abundant, oil-body, metabolism, stress, cellular, protein storage, metabolism, and defense. Defense proteins were up-regulated in response to infection in both CO441 and CL30 lines. Furthermore, F....
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Authors: 3
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