Top 10 Biorxiv Papers Today in Plant Biology


2.088 Mikeys
#1. From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex
Carlos Samuel Galvan-Ampudia, Guillaume Cerutti, Jonathan Legrand, Romain Azais, Géraldine Brunoud, Steven Moussu, Christian Wenzl, Jan Lohmann, Christophe Godin, Teva Vernoux
A key question in developmental biology is how morphogenetic regulators control patterning. Recent findings have raised an important question: do morphogenetic signals carry information not only in space, as originally proposed in the morphogen concept, but also in time? The hormone auxin is an essential plant morphogenetic regulator that drives rhythmic organogenesis at the shoot apical meristem. Here, we used a quantitative imaging approach to map auxin distribution and response. We demonstrate the existence of high-definition spatio-temporal auxin distribution in the meristem. We provide evidence that developing organs are auxin-emitting centers that could allow self-sustained distribution of auxin through a structured auxin transport network converging on the meristem center. We finally demonstrate that regulation of histone acetylation allows cells to measure the duration of the exposition to auxin preceding organ initiation, providing a remarkable example of how both spatial and temporal morphogenetic information generates...
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biorxivpreprint: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/Bu5MM6L9HH #bioRxiv
biorxiv_plants: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/QLoWBlqtcQ #biorxiv_plants
teva_vernoux: All you wanted to know about the dynamics of auxin in the shoot apical meristem and the global regulation of auxin responses: https://t.co/csgQiuBuhP and https://t.co/7qDSxXZoAH ; excellent collaboration with @Meristemania
Zedulias: RT @biorxiv_plants: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/QLoWBlqtcQ #biorxiv…
Andrea_AGF89: RT @biorxiv_plants: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/QLoWBlqtcQ #biorxiv…
Lo28321839: RT @biorxiv_plants: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/QLoWBlqtcQ #biorxiv…
MatthieuPlatre: RT @biorxiv_plants: From spatio-temporal morphogenetic gradients to rhythmic patterning at the shoot apex https://t.co/QLoWBlqtcQ #biorxiv…
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Sample Sizes : [3045, 1895, 3378, 5621, 42991, 6003, 69, 21]
Authors: 10
Total Words: 24504
Unqiue Words: 4458

2.027 Mikeys
#2. WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis
Yanfei Ma, Andrej Miotk, Zoran Sutikovic, Anna Medzihradszky, Christian Enzl, Olga Ermakova, Christophe Gaillochet, Joachim Forner, Goezde Utan, Klaus Brackmann, Carlos Galvan-Ampudia, Teva Vernoux, Greb Thomas, Jan U. Lohmann
During development and growth, dynamic signals need to be translated into spatially precise and temporally stable gene expression states, which define cell fate. In the context of the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers organ initiation. Here, we show that the WUSCHEL transcription factor locally protects stem cells from differentiation by controlling the auxin signaling and response pathway via regulation of histone acetylation. Conversely, low levels of signaling are required for stem cell maintenance, demonstrating that WUSCHEL acts as a rheostat on the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly mobile developmental signal into appropriate cell behavior with high spatial precision and temporal robustness.
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Meristemania: Auxin is all over the SAM, but only some cells respond. Two preprints addressing this long standing question from @teva_vernoux and us. Spoiler: chromatin acetylation may be involved. https://t.co/NCWbb7uQhi https://t.co/UDz4Fq93Y6
ApolabL: Interesting paper about auxin dynamics in apical stem cells https://t.co/aQGf9VoCo6
PlantoPhagy: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
MoritzNowack: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
Dornomics: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
eirini_kaiserli: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
So_it_flows: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
TomRegnault: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
GioPerrella26: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
Prashanth_UU: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
MatthieuPlatre: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
David_G_M_Bio: RT @biorxiv_plants: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis https://t.co/RcQ17HbRLB #…
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Sample Sizes : [90, 30]
Authors: 14
Total Words: 10973
Unqiue Words: 3552

2.017 Mikeys
#3. A simple method to co-purify genomic DNA, RNA, and proteins for functional studies
Jian Jiang, Junfei Ma, Bin Liu, Ying Wang
Understanding the regulation of gene expression, from the epigenetic modifications on genomes to posttranscriptional and translational controls, are critical for elucidating molecular mechanisms underlying distinct phenotypes in biology. With the rapid development of Multi-Omics analyses, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on Tri Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantity, such as protoplasts. We demonstrated that the protoplast system equipped with this method may facilitate studies on viroid biogenesis. Given the ease and the robustness of this new method, it will have broad...
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ProtifiLlc: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/IfnMEqeoWy
PromPreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/38PSChTVx8
Deepak_ddb: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/vCleemvDDb
IMPvienna: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
mtanichthys: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
phdilly: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
SelvarajGuru: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
ProteomicsNews: RT @ProtifiLlc: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/IfnMEqeoWy
BejaLab: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Varsani_lab: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
rravi: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
genvlp: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
manueltard: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
daforerog: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
aeRIsincOsmos: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
tSILIChtetL: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
tingfordha: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Vaikuntapu_etal: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
PankajTri29: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
analioj: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
ImmunityForever: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
mehrshmali: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
eirini_kaiserli: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
Scedacity: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
ranflab: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
PrecursorCell: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Juli_Bla: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
pbrachova: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Uraeus_Nefer: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
gzinta: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
IlottNick: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
bioinfo_mark: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
SbastienGauvri1: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Mfitzg79Mark: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
BleinThomas: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
Vallecillo_AJ: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
shaman_ns: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
vimavi8: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
gsava3: RT @ProtifiLlc: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/IfnMEqeoWy
GioPerrella26: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
FabianBraukmann: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Chris_G_Smith1: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
In_AnkitSingla: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
ji_timedreamer: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
Masayuki_Horie: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
mileguerrerof: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
lishuai800: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
Anchilie: RT @biorxiv_plants: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/1xJJm3Pufr #biorxiv_pl…
Geet_05: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
AshleyBWilliam4: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
AleXDiGiOX: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
kororoad1: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
SwapnaBioinfo: RT @biorxivpreprint: A simple method to co-purify genomic DNA, RNA, and proteins for functional studies https://t.co/GWhF05Cqjb #bioRxiv
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Sample Sizes : None.
Authors: 4
Total Words: 7249
Unqiue Words: 2162

1.998 Mikeys
#4. Spatiotemporal expression of FRIGIDA modulate flowering time in Arabidopsis thaliana
Xiangxiang Kong, Jinjie Zhao, Landi Luo, Qian Chen, Guanxiao Chang, Jingling Huang, Yongping Yang, Xiangyang Hu
FRIGIDA (FRI) as the major regulator of flowering time in Arabidopsis accessions can activate its target FLOWERING LOCUS C (FLC) to delay flowering before vernalization. Besides FLC, other FRI targets also exist in Arabidopsis. Although leaves sense environmental cues to modulate flowering time, it is not known if roots also regulate the floral transition. In this study, we investigated the spatiotemporal effect of FRI on flowering time. Local expression of FRI in the phloem and leaves activated FLC to delay flowering. Furthermore, we found that local expression of FRI in the roots also delayed flowering by activating other targets MADS AFFECTING FLOWERING4 (MAF4) and MAF5 in the roots. Graft and genetic experiments revealed that the spatial expression of FRI in the root might generate a mobile signal, which is transmitted from roots to shoot and antagonizes the FT signal to delay flowering. Specifically expressing FRI in the embryo efficiently delayed flowering, even expressing FRI as early as pro-embryo stage is enough to...
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SelvarajGuru: RT @biorxiv_plants: Spatiotemporal expression of FRIGIDA modulate flowering time in Arabidopsis thaliana https://t.co/tTeUaX6ZFv #biorxiv_…
Dornomics: RT @biorxiv_plants: Spatiotemporal expression of FRIGIDA modulate flowering time in Arabidopsis thaliana https://t.co/tTeUaX6ZFv #biorxiv_…
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Sample Sizes : None.
Authors: 8
Total Words: 11822
Unqiue Words: 2583

1.996 Mikeys
#5. Yield potential definition of the chilling requirement reveals likely underestimation of the risk of climate change on winter chill accumulation
Jose Antonio Campoy, Rebecca Darbyshire, Elisabeth Dirlewanger, Jose Quero-Garcia, Benedicte Wenden
Evaluation of chilling requirements of cultivars of temperate fruit trees provides key information to assess regional suitability, according to winter chill, for both industry expansion and ongoing profitability as climate change progresses. Traditional methods for calculating chilling requirements use climate controlled chambers and define chilling requirements using a fixed bud burst percentage, usually close to 50% (CR-50%). However, this CR-50% definition may estimate chilling requirements that lead to flowering percentages that are lower than required for orchards to be commercially viable. We used sweet cherry to analyse the traditional method for calculating chilling requirements (CR-50%) and compared the results with a more restrictive method, where the chilling requirement was defined by a 90% bud break level (CRm-90%). For sweet cherry, this higher requirement of flowering success (90% as opposed to 50%) better represents grower production needs as a greater number of flowers lead to greater potential yield. To...
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Authors: 5
Total Words: 7425
Unqiue Words: 2469

0.0 Mikeys
#6. A Genome-Wide Association Study Reveals a Novel Regulator of Ovule Number and Fertility in Arabidopsis thaliana
Jing Yuan, Sharon A Kessler
Ovules contain the female gametophytes which are fertilized during pollination to initiate seed development. Thus, the number of ovules that are produced during flower development is an important determinant of seed crop yield and plant fitness. Mutants with pleiotropic effects on development often alter the number of ovules, but specific regulators of ovule number have been difficult to identify in traditional mutant screens. We used natural variation in Arabidopsis accessions to identify new genes involved in the regulation of ovule number. The ovule numbers per flower of 189 Arabidopsis accessions were determined and found to have broad phenotypic variation that ranged from 39 ovules to 84 ovules per pistil. Genome-Wide Association tests revealed several genomic regions that are associated with ovule number. T-DNA insertion lines in candidate genes from the most significantly associated loci were screened for ovule number phenotypes. The NEW ENHANCER of ROOT DWARFISM (NERD1) gene was found to have pleiotropic effects on plant...
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Authors: 2
Total Words: 9962
Unqiue Words: 2801

0.0 Mikeys
#7. Seed Genome Hypomethylated Regions Are Enriched In Transcription Factor Genes
Min Chen, Jer-Young Lin, Jungim Hur, Julie M Pelletier, Russell Baden, Matteo Pellegrini, John J Harada, Robert Goldberg
The precise mechanisms that control gene activity during seed development remain largely unknown. Previously, we showed that several genes essential for seed development, including those encoding storage proteins, fatty acid biosynthesis enzymes, and transcriptional regulators, such as ABI3 and FUS3, are located within hypomethylated regions of the soybean genome. These hypomethylated regions are similar to the DNA methylation valleys (DMVs), or canyons, found in mammalian cells. Here, we address the question of the extent to which DMVs are present within seed genomes, and what role they might play in seed development. We scanned soybean and Arabidopsis seed genomes from post-fertilization through dormancy and germination for regions that contain < 5% or < 0.4% bulk methylation in CG-, CHG-, and CHH-contexts over all developmental stages. We found that DMVs represent extensive portions of seed genomes, range in size from 5 to 76 kb, are scattered throughout all chromosomes, and are hypomethylated throughout the plant life cycle....
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biorxivpreprint: Seed Genome Hypomethylated Regions Are Enriched In Transcription Factor Genes https://t.co/8RLsbpjNZe #bioRxiv
biorxiv_plants: Seed Genome Hypomethylated Regions Are Enriched In Transcription Factor Genes https://t.co/ixnHMJIwcN #biorxiv_plants
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Authors: 8
Total Words: 12053
Unqiue Words: 2974

0.0 Mikeys
#8. Thylakoid-integrated recombinant Hcf106 participates in the chloroplast Twin Arginine Transport (cpTat) system
Qianqian Ma, Kristen Fite, Christopher Paul New, Carole Dabney-Smith
The chloroplast Twin arginine transport (cpTat) system distinguishes itself as a protein transport pathway by translocating fully-folded proteins, using the proton-motive force (PMF) as the sole source of energy. The cpTat pathway is evolutionarily conserved with the Tat pathway found in the plasma membrane of many prokaryotes. The cpTat (E. coli) system uses three proteins, Tha4 (TatA), Hcf106 (TatB), and cpTatC (TatC), to form a transient translocase allowing the passage of precursor proteins. Briefly, cpTatC and Hcf106, with Tha4, form the initial receptor complex responsible for precursor protein recognition and binding in an energy-independent manner, while a separate pool of Tha4 assembles with the precursor-bound receptor complex in the presence the PMF. Analysis by blue-native polyacrylamide gel electrophoresis (BN-PAGE) shows that the receptor complex, in the absence of precursor, migrates near 700 kDa and contains cpTatC and Hcf106 with little Tha4 remaining after detergent solubilization. To investigate the role that...
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SecretionPapers: Thylakoid-integrated recombinant Hcf106 participates in the chloroplast #Tat (cpTat) system @c_dabsinscience @biorxivpreprint https://t.co/rc9fcN3YKJ
c_dabsinscience: Thylakoid-integrated recombinant Hcf106 participates in the chloroplast Twin Arginine Transport (cpTat) system https://t.co/svPlHFgHWf
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Authors: 4
Total Words: 10321
Unqiue Words: 3232

0.0 Mikeys
#9. The RopGEF KARAPPO is Essential for the Initiation of Vegetative Reproduction in Marchantia
Takuma Hiwatashi, Koh Li Quan, Yukiko Yasui, Hideyuki Takami, Masataka Kajikawa, Hiroyuki Kirita, Mayuko Sato, Mayumi Wakazaki, Katsushi Yamaguchi, Shuji Shigenobu, Hidehiro Fukaki, Tetsuro Mimura, Katsuyuki T. Yamato, Kiminori Toyooka, Shinichiro Sawa, Daisuke Urano, Takayuki Kohchi, Kimitsune Ishizaki
Many plants can reproduce vegetatively, producing clonal progeny from vegetative cells; however, little is known about the molecular mechanisms underlying this process. Liverwort ( Marchantia polymorpha ), a basal land plant, propagates asexually via gemmae, which are clonal plantlets formed in gemma cups on the dorsal side of the vegetative thallus. The initial stage of gemma development involves elongation and asymmetric divisions of a specific type of epidermal cell, called a gemma initial, which forms on the floor of the gemma cup. To investigate the regulatory mechanism underlying gemma development, we focused on two allelic mutants in which no gemma initial formed; these mutants were named karappo , meaning 'empty'. We used whole-genome sequencing of both mutants, and molecular genetic analyses to identify the causal gene, KARAPPO ( KAR ), which encodes a Rop guanine nucleotide exchange factor (RopGEF) carrying a PRONE catalytic domain. In vitro GEF assays showed that the full-length KAR protein and the PRONE domain have...
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Phil_Carella: Check out these #Marchantia mutants with empty cups! "The RopGEF KARAPPO is Essential for the Initiation of Vegetative Reproduction in Marchantia" https://t.co/F9JBEuzjDI
dolfweijers: RT @biorxiv_plants: The RopGEF KARAPPO is Essential for the Initiation of Vegetative Reproduction in Marchantia https://t.co/gfuHGWJP5L #b…
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Authors: 18
Total Words: 11925
Unqiue Words: 3986

0.0 Mikeys
#10. Light dynamically regulates growth rate and cellular organisation of the Arabidopsis root meristem.
Thomas Blein, Jasmin Duerr, Taras Pasternak, Thomas Haser, Thorsten Falk, Kun Liu, Franck Anicet Ditengou, Olaf Ronneberger, Klaus Palme
Large-scale methods and robust algorithms are needed for a quantitative analysis of cells status/geometry in situ. It allows the understanding the cellular mechanisms that direct organ growth in response to internal and environmental cues. Using advanced whole-stack imaging in combination with pattern analysis, we have developed a new approach to investigate root zonation under different dark/light conditions. This method is based on the determination of 3 different parameters: cell length, cell volume and cell proliferation on the cell-layer level. This method allowed to build a precise quantitative 3D cell atlas of the Arabidopsis root tip. Using this approach we showed that the meristematic (proliferation) zone length differs between cell layers. Considering only the rapid increase of cortex cell length to determine the meristematic zone overestimates of the proliferation zone for epidermis/cortex and underestimates it for pericycle. The use of cell volume instead of cell length to define the meristematic zone correlates better...
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Sample Sizes : [16]
Authors: 9
Total Words: 12543
Unqiue Words: 2872

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