Top 8 Biorxiv Papers Today in Plant Biology


1.998 Mikeys
#1. Role of Cytosolic, Tyrosine-Insensitive Prephenate Dehydrogenase in Medicago truncatula
Craig A Schenck, Josh Westphal, Dhileepkumar Jayaraman, Kevin Garcia, Jiangqi Wen, Kirankumar S Mysore, Jean-Michel Aneé, Lloyd W Sumner, Hiroshi A Maeda
L-Tyrosine (Tyr) is an aromatic amino acid synthesized de novo in plants and microbes downstream of the shikimate pathway. In plants, Tyr and a Tyr pathway intermediate, 4-hydroxyphenylpyruvate (HPP), are precursors to numerous specialized metabolites, which are crucial for plant and human health. Tyr is synthesized in the plastids by a TyrA family enzyme, arogenate dehydrogenase (ADH/TyrAa), which is feedback inhibited by Tyr. In addition to ADH enzymes, many legumes possess prephenate dehydrogenases (PDH/TyrAp), which are insensitive to Tyr and localized to the cytosol. Yet the role of PDH in legumes is currently unknown. This study isolated and characterized Tnt1 -transposon mutants of MtPDH1 ( pdh1 ) in Medicago truncatula to investigate PDH function. The pdh1 mutants lacked PDH transcript, PDH activity, and displayed little aberrant morphological phenotypes under standard growth conditions providing genetic evidence that MtPDH1 is responsible for the PDH activity detected in M. truncatula . Though plant PDH enzymes and...
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biorxivpreprint: Role of Cytosolic, Tyrosine-Insensitive Prephenate Dehydrogenase in Medicago truncatula https://t.co/RZXnW5LqrV #bioRxiv
biorxiv_plants: Role of Cytosolic, Tyrosine-Insensitive Prephenate Dehydrogenase in Medicago truncatula https://t.co/Fkd3v5TjZy #biorxiv_plants
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Authors: 9
Total Words: 14286
Unqiue Words: 4670

1.998 Mikeys
#2. High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification
Hanna Help, Merja Lusa, Ari-Pekka Honkanen, Ana Diaz, Mirko Holler, Murielle Salome, Peter Cloetens, Henrik Makinen, Simo Huotari, Heikki Suhonen
Plant-microbe interactions can be utilized in bio-based processes such as bioremediation and bio-fortification, either to remove hazardous radionuclides and heavy metals from the soil, or to increase the accumulation of desired elements into crops to improve their quality. Optimizing such elegant plant-microbe interactions requires detailed understanding of the chemical element compositions of fresh plant tissues at cellular organelle resolution. However, such analyses remain challenging because conventional methods lack the required spatial resolution, contrast or sensitivity. Using a novel combination of nanoscale-resolution 3D cryogenic synchrotron-light ptychography, holotomography and fluorescence tomography, we show how soil bacteria interact with Arabidopsis thaliana and promote the uptake of various metals. Co-cultivation with Pseudomonas sp. strain T5-6-I alters root anatomy and increases levels of selenium (Se), iron (Fe) and other micro nutrients in roots. Our approach highlights the interaction of plants and microbes...
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biorxivpreprint: High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification https://t.co/7K89xZQjci #bioRxiv
biorxiv_plants: High-resolution synchrotron imaging studies of intact fresh roots reveal soil bacteria promoted bioremediation and bio-fortification https://t.co/sfbHs39tVL #biorxiv_plants
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Authors: 10
Total Words: 16449
Unqiue Words: 5291

1.998 Mikeys
#3. DCT4 - a new member of the dicarboxylate transporter family in C4 grasses.
Sarit Weissmann, Pu Huang, Koki Furuyama, Madeline Wiechert, Mitsutaka Taniguchi, James Schnable, Thomas Brutnell, Todd C Mockler
Malate transport shuttles atmospheric carbon into the Calvin-Benson cycle during NADP-ME C4 photosynthesis. Previous characterizations of several plant dicarboxylate transporters (DCT) showed that they efficiently exchange malate across membranes. Here we identify and characterize a previously unknown member of the DCT family, DCT4, in Sorghum bicolor. We show that SbDCT4 exchanges malate across membranes and its expression pattern is consistent with a role in malate transport during C4 photosynthesis. SbDCT4 is not syntenic to the characterized photosynthetic gene ZmDCT2, and an ortholog is not detectable in the maize reference genome. We found that the expression patterns of DCT family genes in the leaves of Z. mays, and S. bicolor varied by cell type. Our results suggest that sub-functionalization of members of the DCT family for the transport of malate into the bundle sheath (BS) plastids occurred during the process of independent recurrent evolution of C4 photosynthesis in grasses of the PACMAD clade. This study confirms the...
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biorxivpreprint: DCT4 - a new member of the dicarboxylate transporter family in C4 grasses. https://t.co/5iBmV2ORRc #bioRxiv
biorxiv_plants: DCT4 - a new member of the dicarboxylate transporter family in C4 grasses. https://t.co/Hry7ZoIq4p #biorxiv_plants
MRT_quasiPhD: RT @biorxiv_plants: DCT4 - a new member of the dicarboxylate transporter family in C4 grasses. https://t.co/Hry7ZoIq4p #biorxiv_plants
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Authors: 8
Total Words: 6313
Unqiue Words: 1944

1.998 Mikeys
#4. A new strategy for selection of crop cultivated varieties based on yield factors driving and meteorological prediction
Rongsheng Zhu, Kai Sun, Xuehui Yan, Dawei Xin, Hongwei Jiang, Zhanguo Zhang, Yang Li, Zhenbang Hu, Zhaoming Qi, Chunyan Liu, Qingshan Chen
Meteorological conditions, as an uncontrollable factor, have a direct impact on the growth and yield of crops in the field. It is particularly important to choose the cultivated varieties suitable for environmental change. As for this problem, a new strategy for selection of crop cultivated varieties is proposed in this paper. The experiment selected Harbin pilot (Heilongjiang, China) and Changchun pilot (Jilin, China). In the experiment, the cultivated varieties suitable for forecasting meteorological conditions in 2015 could be provided according to the meteorological data of 2005-2014 years and the yield data of different soybean varieties in Harbin pilot. The same method was also taken in Changchun pilot. The results showed that the recommended varieties of Harbin pilot and Changchun pilot had 70% and 50% probability respectively for selection of soybean cultivated varieties when the yield evaluation trait was 100-seed-weight. As for seed weight per plant, the possibility of more than 60% of the recommended varieties in two...
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Authors: 11
Total Words: 0
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1.997 Mikeys
#5. Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid
Joram A. Dongus, Jyoti Shah, Lucas Dijkgraaf, Lani Archer, Laurent Deslandes, Monika Patel, Deepak D. Bhandari, Jane E. Parker
Plants have evolved mechanisms to attract beneficial microbes and insects while protecting themselves against pathogenic microbes and pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by Green Peach Aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4-lipase-like domain (LLD) without its C-terminal "EDS1-PAD4" (EP) domain, we interrogated PAD4 functions in plant defense. Here we show that transgenic expression of PAD4LLD in Arabidopsis is sufficient for limiting GPA infestation, but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4-EP domain is necessary for EDS1-dependent immune functions. Moreover, PAD4LLD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for heterodimerisation. These data provide molecular evidence that PAD4 has domain specific functions.
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Authors: 8
Total Words: 0
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1.997 Mikeys
#6. Sex Lability and Dimorphism in Diecious Palmer Amaranth (Amaranthus palmeri)
Mohsen B. Mesgaran, Maor Matzrafi, Sara Ohadi
Dioecious weeds (separate sexes) may benefit from a maximized outcrossing and optimal sex-specific resource allocation but there are costs associated with the evolution of this breeding system which can be exploited for long-term management of dioecious weeds. That is, seed production in dioecious species is contingent upon the co-occurrence and co-flowering of the two genders and can be further complicated by biases in sex ratio. We therefore explored the sex ratio and dimorphism in secondary sex characters in three populations of Palmer amaranth (Amaranthus palmeri) from California, Kansas and Texas and tested if water stress can change the sex expression and/or the synchrony of flowering (anthesis) between male and female plants. Sex ratio (proportion of males) was balanced and did not deviate from 1:1 in all experiments and populations (California, Kansas, Texas) when plants received normal watering. Male and female plants of A. palmeri did not differ in timing of emergence, plant height and relative growth rate. While the...
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biorxivpreprint: Sex Lability and Dimorphism in Diecious Palmer Amaranth (Amaranthus palmeri) https://t.co/rtxs0pXBdW #bioRxiv
biorxiv_plants: Sex Lability and Dimorphism in Diecious Palmer Amaranth (Amaranthus palmeri) https://t.co/dgu4dBiZWO #biorxiv_plants
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Sample Sizes : [200]
Authors: 3
Total Words: 8175
Unqiue Words: 2657

1.997 Mikeys
#7. Minimally invasive, pressure probe based sampling allows for in-situ gene expression analyses in plant cells.
Hiroshi Wada, Simone D. Castellarin, Mark A. Matthews, Kenneth A. Shackel, Gregory A. Gambetta
Background: Gene expression analyses are conducted using multiple approaches and increasingly research has been focused on assessing gene expression at the level of a tissue or even single-cells. To date, methods to assess gene expression at the single-cell in plant tissues have been semi-quantitative, require tissue disruption, and/or involve laborious, possibly artifact-inducing manipulation. In this work, we used grape berries ( Vitis vinifera L. Zinfandel) as a model in order to examine the validity and reproducibility of an in-situ gene expression analysis method combining a cell pressure probe (CPP) with quantitative PCR (qPCR). Results: We developed a method to directly assess gene expression levels via qPCR from cellular fluids sampled in-situ with a CPP. Cellular fluids, with volumes in the picoliter range, were collected from intact berries with a CPP at various depths across skin and mesocarp tissues. The expression of a key anthocyanin biosynthetic gene, UDP-glucose: flavonoid 3-O-glucosyltransferase ( VviUFGT ), was...
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biorxivpreprint: Minimally invasive, pressure probe based sampling allows for in-situ gene expression analyses in plant cells. https://t.co/PxdAzlwKU1 #bioRxiv
biorxiv_plants: Minimally invasive, pressure probe based sampling allows for in-situ gene expression analyses in plant cells. https://t.co/R1bXV2e6mr #biorxiv_plants
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Authors: 5
Total Words: 0
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1.997 Mikeys
#8. Arabidopsis thaliana natural variation in temperature-modulated immunity uncovers transcription factor UNE12 as a thermoresponsive regulator
Friederike Bruessow, Jaqueline Bautor, Gesa Hoffmann, Jane E Parker
Temperature impacts plant immunity and growth but how temperature intersects with endogenous pathways remains unclear. Here we uncover variation between Arabidopsis thaliana natural accessions in response to two non-stress temperatures (22°C and 16°C) affecting accumulation of the thermoresponsive stress hormone salicylic acid (SA) and plant growth. Analysis of differentially responding A. thaliana accessions shows that pre-existing SA provides a benefit in limiting bacterial pathogen infection at both temperatures. Several A. thaliana genotypes display a capacity to mitigate negative effects of high SA on growth, indicating within-species plasticity in SA - growth tradeoffs. An association study of temperature x SA variation, followed by physiological and immunity phenotyping of mutant and over-expression lines, identifies the transcription factor unfertilized embryo sac 12 (UNE12) as a temperature-responsive SA immunity regulator. Here we reveal previously untapped diversity in plant responses to temperature and a way forward in...
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Authors: 4
Total Words: 14388
Unqiue Words: 4346

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