Top 4 Biorxiv Papers Today in Physiology


2.025 Mikeys
#1. Intraperitoneal injection of sodium pentobarbital is associated with pain in rats.
Julie Reimer, Chelsea Schuster, Cameron G Knight, Daniel Pang, Vivian SY Leung
An effective and pain-free killing method is required to achieve the goal of euthanasia, a “good death”. Overdose of sodium pentobarbital (PB) by intraperitoneal (IP) injection is a widely accepted technique, but questions remain regarding pain associated with administration. As PB rapidly causes sedation and loss of consciousness, most studies have relied on indirect evidence of pain. The objective of this study was to assess pain associated with IP PB using an appropriate vehicle control. Adult male and female Sprague Dawley (SD) and female Wistar rats (N = 112) were block randomised by sex and strain to receive one of four treatments: 1) 800 mg/kg PB (pH 11); 2) 800 mg/kg PB with 4 mg/kg lidocaine (PB+lido); 3) saline or 4) vehicle controls (pH 11 or 12.5). Behavior (Rat Grimace Scale [RGS], writhing, back arching) was evaluated at baseline, before loss of righting reflex (PB and PB+lido groups), 80s, 151s and 10 min post-injection (PI; saline and vehicle control groups). In the vehicle control groups, the RGS scores were...
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biorxivpreprint: Intraperitoneal injection of sodium pentobarbital is associated with pain in rats. https://t.co/RhQga1zQG1 #bioRxiv
biorxiv_physio: Intraperitoneal injection of sodium pentobarbital is associated with pain in rats. https://t.co/zYauvp5Mfm #biorxiv_physio
Umasi1981: RT @biorxivpreprint: Intraperitoneal injection of sodium pentobarbital is associated with pain in rats. https://t.co/RhQga1zQG1 #bioRxiv
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Authors: 5
Total Words: 7055
Unqiue Words: 2454

2.009 Mikeys
#2. Oxygen supply capacity in animals evolves to meet maximum demand at the current oxygen partial pressure regardless of size or temperature
Brad A Seibel, Curtis Deutsch
Physiological oxygen supply capacity is associated with athletic performance and cardiovascular health and is thought to cause hypometabolic scaling in diverse species. Environmental oxygen is widely believed to be limiting of metabolic rate and aerobic scope, setting thermal tolerance and body size limits with implications for species diversity and biogeography. Here we derive a quantifiable linkage between maximum and basal metabolic rate and their temperature, size and oxygen dependencies. We show that, regardless of size or temperature, the capacity for oxygen supply precisely matches the maximum evolved demand at the highest persistently available oxygen pressure which, for most species assessed, is the current atmospheric pressure. Any reduction in oxygen partial pressure from current values will result in a decrement in maximum metabolic performance. However, oxygen supply capacity does not constrain thermal tolerance and does not cause hypometabolic scaling. The critical oxygen pressure, typically viewed as an indicator of...
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biorxivpreprint: Oxygen supply capacity in animals evolves to meet maximum demand at the current oxygen partial pressure regardless of size or temperature https://t.co/clWPbn1zce #bioRxiv
biorxiv_physio: Oxygen supply capacity in animals evolves to meet maximum demand at the current oxygen partial pressure regardless of size or temperature https://t.co/2PAyUh75mi #biorxiv_physio
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Authors: 2
Total Words: 11803
Unqiue Words: 4001

1.998 Mikeys
#3. A missense mutation dissociates triglyceride and phospholipid transfer activities in zebrafish and human microsomal triglyceride transfer protein
Meredith H Wilson, Sujith Rajan, Aidan Danoff, Richard J. White, Monica R. Hensley, Vanessa H. Quinlivan, James H. Thierer, Elisabeth M. Busch-Nentwich, M. Mahmood Hussain, Steven A. Farber
Microsomal triglyceride transfer protein (MTP) transfers triglycerides and phospholipids and is essential for the assembly of Apolipoprotein B (ApoB)-containing lipoproteins in the endoplasmic reticulum. We have discovered a zebrafish mutant ( mttpc655 ) expressing a C-terminal missense mutation (G863V) in Mttp, one of the two subunits of MTP, that is defective at transferring triglycerides, but retains phospholipid transfer activity. Mutagenesis of the conserved glycine in the human MTTP protein (G865V) also eliminates triglyceride but not phospholipid transfer activity. The G863V mutation reduces the production and size of ApoB-containing lipoproteins in zebrafish embryos and results in the accumulation of cytoplasmic lipid droplets in the yolk syncytial layer. However, mttpc655 mutants exhibit only mild intestinal lipid malabsorption and normal growth as adults. In contrast, zebrafish mutants bearing the previously identified mttpstl mutation (L475P) are deficient in transferring both triglycerides and phospholipids and exhibit...
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Authors: 10
Total Words: 21210
Unqiue Words: 6586

1.996 Mikeys
#4. The MRL/MpJ Mouse Strain Is Not Protected From Muscle Atrophy And Weakness After Rotator Cuff Tear
Jeffrey R Talarek, Alex N Piacentini, Alexis C Konja, Susumu Wada, Jacob B Swanson, Samuel C Nussenzweig, Joshua S Dines, Scott A Rodeo, Christopher L Mendias
Chronic rotator cuff tears are a common source of shoulder pain and disability. Patients with rotator cuff tears often have substantial weakness, fibrosis, and fat accumulation which limit successful surgical repair and postoperative rehabilitation. The Murphy Roths Large (MRL) strain of mice have demonstrated superior healing and protection against pathological changes in several disease and injury conditions. We tested the hypothesis that, compared to the commonly used C57Bl/6 (B6) strain, MRL mice would have less muscle fiber atrophy and fat accumulation, and be protected against the loss in force production that occurs after cuff tear. Adult male mice B6 and MRL mice were subjected to a rotator cuff tear, and changes in muscle fiber contractility and histology were measured. RNA sequencing, and shotgun metabolomics and lipidomics were also performed. Muscles were harvested one month after tear. B6 and MRL mice had a 40% reduction in relative muscle force production after rotator cuff tear. RNA sequencing identified an increase...
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Authors: 9
Total Words: 0
Unqiue Words: 0

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