Top 6 Biorxiv Papers Today in Molecular Biology


1.998 Mikeys
#1. RAD51 promotes non-homologous genetic rearrangements that are prevented by 53BP1
Josée Guirouilh-Barbat, Wei Yu, Loelia Babin, Elisa Yaniz Galende, Tatiana Popova, Ayeong So, Chloé Lescale, Marc-Henri Stern, Erika Brunet, Ludovic Deriano, Bernard S Lopez
Homologous recombination (HR), which requires long sequence homologies, is considered a high fidelity mechanism, preserving genome stability. In contrast, we show here that the central HR players RAD51 or BRCA2, promote genetic instability, fostering translocations and capture of ectopic chromosomal sequences when joining distant DNA breaks. Surprisingly, these events do not involve sequence homologies. Moreover, our data reveal that 53BP1 protects against RAD51-mediated non-homologous genetic rearrangements. Finally, analysis of a large panel of breast tumors revealed that BRCA2 proficiency is associated with increased frequency of capture of non-homologous sequences at junctions of structural variants (translocations, duplications, inversions, deletions). These data reveal that HR proteins (RAD51, BRCA2) possess the intrinsic capacity to generate genetic instability through sequence homology-independent processes, and that 53BP1 protects against it. We propose that BRCA2/RAD51-mediated genome instability occurs in the course of...
more | pdf
Figures
Tweets
biorxivpreprint: RAD51 promotes non-homologous genetic rearrangements that are prevented by 53BP1 https://t.co/CYIah1YYFH #bioRxiv
NeedhiBhalla: RT @biorxivpreprint: RAD51 promotes non-homologous genetic rearrangements that are prevented by 53BP1 https://t.co/CYIah1YYFH #bioRxiv
enerphyschem: RT @biorxivpreprint: RAD51 promotes non-homologous genetic rearrangements that are prevented by 53BP1 https://t.co/CYIah1YYFH #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 11
Total Words: 11181
Unqiue Words: 3200

1.998 Mikeys
#2. Alternative catalytic residues in the active site of Esco acetyltransferases
Tahereh Ajam, Inessa De, Nikolai Petkau, Gabriela Whelan, Vladimir Pena, Gregor Eichele
Cohesin is a protein complex that encircles the DNA and regulates the separation of sister chromatids during cell division. Following a catalytic mechanism that is insufficiently understood, Esco1 and Esco2 acetyltransferases acetylate the cohesin subunit Smc3, thereby inducing a stabilization of cohesin on DNA. As a prerequisite for structure-guided investigation of enzymatic activity, we determine here the crystal structure of the mouse Esco2/CoA complex at 1.8 Å resolution. We reconstitute the entire cohesin as a tetrameric assembly and use it as a physiologically-relevant substrate for enzymatic assays in vitro. Furthermore, we employ cell-based complementation studies in mouse embryonic fibroblast deficient for Esco1 and Esco2, as a means to identify catalytically-important residues in vivo. These analyses demonstrate that D567/S566 and E491/S527, located on opposite sides of the MmEsco2 active site cleft, are critical for catalysis. Our experiments supports a catalytic mechanism of acetylation where residues D567 and E491...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : [2, 3]
Authors: 6
Total Words: 10098
Unqiue Words: 2785

1.997 Mikeys
#3. DNA repair by Rad52 liquid droplets
Roxanne Oshidari, Richard Huang, Maryam Medghalchi, Elizabeth Y. W. Tse, Nasser Ashgriz, Hyun O. Lee, Haley Wyatt, Karim Mekhail
Cellular processes are influenced by liquid phase separation, but its role in DNA repair is unclear. Here, we show that in Saccharomyces cerevisiae, Rad52 DNA repair proteins at different DNA damage sites assemble liquid droplets that fuse into a repair centre droplet. This larger droplet concentrates tubulin and projects short aster-like microtubule filaments, which tether the droplet to longer microtubule filaments mediating the mobilization of damaged DNA to the nuclear periphery for repair.
more | pdf
Figures
Tweets
biorxivpreprint: DNA repair by Rad52 liquid droplets https://t.co/vKKzB6XeGt #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 8
Total Words: 4320
Unqiue Words: 1685

1.997 Mikeys
#4. A VPS13D spastic ataxia mutation disrupts the conserved adaptor binding site in yeast Vps13
Samantha K. Dziurdzik, Björn D.M. Bean, Michael Davey, Elizabeth Conibear
Mutations in each of the four human VPS13 (VPS13A-D) proteins are associated with distinct neurological disorders: chorea-acanthocytosis, Cohen syndrome, early-onset Parkinson's disease and spastic ataxia. Recent evidence suggests that the different VPS13 paralogs transport lipids between organelles at different membrane contact sites. How each VPS13 isoform is targeted to organelles is not known. We have shown that the localization of yeast Vps13 protein to membranes requires a conserved six-repeat region, the Vps13 Adaptor Binding (VAB) domain, which binds to organelle-specific adaptors. Here, we use a systematic mutagenesis strategy to determine the role of each repeat in recognizing each known adaptor. Our results show that mutation of invariant asparagines in repeats 1 and 6 strongly impact the binding all adaptors and block Vps13 membrane recruitment. However, we find that repeats 5 to 6 are sufficient for localization and interaction with adaptors. This supports a model where a single adaptor binding site is found in the...
more | pdf
Figures
Tweets
biorxivpreprint: A VPS13D spastic ataxia mutation disrupts the conserved adaptor binding site in yeast Vps13 https://t.co/bUH62Pj1kt #bioRxiv
HenneLab: RT @biorxivpreprint: A VPS13D spastic ataxia mutation disrupts the conserved adaptor binding site in yeast Vps13 https://t.co/bUH62Pj1kt #…
OKaneCahir: RT @biorxivpreprint: A VPS13D spastic ataxia mutation disrupts the conserved adaptor binding site in yeast Vps13 https://t.co/bUH62Pj1kt #…
Github
None.
Youtube
None.
Other stats
Sample Sizes : [3]
Authors: 4
Total Words: 10467
Unqiue Words: 2972

1.997 Mikeys
#5. The B. subtilis Rok protein compacts and organizes DNA by bridging
Liang Qin, Amanda Monique Erkelens, Dorijn Markus, Remus T Dame
Rok from Bacillus subtilis is an abundant DNA binding protein similar in function to H-NS-like proteins found in many proteobacteria. Rok binds across the genome with a preference for A/T rich DNA. Such DNA often contains genes of foreign origin that are silenced due to Rok binding. Rok also has been implied in global organization of the B. subtilis genome. However, how Rok binds to DNA and how it represses transcription is unclear. Also, it is unknown whether Rok-mediated gene repression can be induced or relieved following changes in physico-chemical conditions, as noted for H-NS-like proteins. Here we investigate the DNA binding properties of Rok and determine the effects of physico-chemical conditions on these properties. We demonstrate that Rok is a DNA bridging protein similar to H-NS like proteins from E. coli (H-NS), Pseudomonas sp. (MvaT) and Mycobacteria (Lsr2). Strikingly, unlike these proteins, the ability of Rok to bridge DNA is not affected by changes in physico-chemical conditions. Not being a direct sensor of such...
more | pdf
Figures
None.
Tweets
biorxivpreprint: The B. subtilis Rok protein compacts and organizes DNA by bridging https://t.co/G7R0kXZvhH #bioRxiv
ZUHassan1: RT @biorxivpreprint: The B. subtilis Rok protein compacts and organizes DNA by bridging https://t.co/G7R0kXZvhH #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 4
Total Words: 0
Unqiue Words: 0

1.997 Mikeys
#6. Control of cartilage homeostasis and osteoarthritis progression by mTORC1/4E-BP/eIF4E axis
Victoria Kolupaeva, Olga Katsara, Mukundan Attur
As world population growing older, burden of age-related conditions soars. One of them is osteoarthritis (OA), a debilitating joint disease with no effective treatment. Articular cartilage degeneration is a central event in OA, and changes in expression of many genes in OA cartilage are well-documented. Still, the specific mechanisms are rarely known. We showed that in OA cartilage the increased abundance of many proteins, including extracellular matrix protein Fibronectin (Fn1) and an orphan nuclear receptor Nr4a1 is translationally controlled and requires inactivation of 4EBP, an inhibitor of cap-dependent translation. Importantly, intra-articular injection of the translation inhibitor 4E1RCat reduces Fn1 and Nr4a upregulation in a rodent OA model and delays cartilage degeneration. Our results support the hypothesis that maintaining proper translational control is an important homeostatic mechanism, the loss of which contributes to OA development.
more | pdf
Figures
None.
Tweets
biorxivpreprint: Control of cartilage homeostasis and osteoarthritis progression by mTORC1/4E-BP/eIF4E axis https://t.co/XKgx4mLCtY #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 3
Total Words: 0
Unqiue Words: 0

About

Assert is a website where the best academic papers on arXiv (computer science, math, physics), bioRxiv (biology), BITSS (reproducibility), EarthArXiv (earth science), engrXiv (engineering), LawArXiv (law), PsyArXiv (psychology), SocArXiv (social science), and SportRxiv (sport research) bubble to the top each day.

Papers are scored (in real-time) based on how verifiable they are (as determined by their Github repos) and how interesting they are (based on Twitter).

To see top papers, follow us on twitter @assertpub_ (arXiv), @assert_pub (bioRxiv), and @assertpub_dev (everything else).

To see beautiful figures extracted from papers, follow us on Instagram.

Tracking 189,566 papers.

Search
Sort results based on if they are interesting or reproducible.
Interesting
Reproducible
Feedback
Online
Stats
Tracking 189,566 papers.