Top 4 Biorxiv Papers Today in Molecular Biology


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#1. Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events
Benjamin Douglas Young, Xaymara Serrano, Stephanie Rosales, Margaret Miller, Dana Williams, Nikki Traylor-Knowles
Coral disease outbreaks are expected to increase in prevalence, frequency and severity due to climate change and other anthropogenic stressors. This is especially worrying for the Caribbean branching Acropora palmata which has already seen an 80% decrease in its coral cover, with this primarily due to disease. Despite the importance of this species, there has yet to be a characterization of its transcriptomic response to disease exposure. In this study we provide the first transcriptomic analysis of 12 A. palmata genotypes, and their symbiont Symbiodiniaceae, exposed to disease in 2016 and 2017. Year was the primary driver of sample variance for A. palmata and the Symbiodiniaceae. Lower expression of ribosomal genes in the coral, and higher expression of transmembrane ion transport genes in the Symbiodiniaceae indicate that the increased virulence in 2017 may have been due to a dysbiosis between the coral and Symbiodiniaceae. We also identified a conserved suite of innate immune genes responding to the disease challenge that was...
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biorxivpreprint: Innate immune gene expression in Acropora palmata is consistent despite variance in yearly disease events https://t.co/Bpy9dyRWRa #bioRxiv
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#2. Genetic diversity evaluation among fifty sugarcane accessions from the Zimbabwe Sugar Industry using DNA-SSR markers and qualitative traits.
Edith Mugehu, Leo Thokoza Mpofu, Pepukai Manjeru
Due to the increasingly diverse needs of the Zimbabwe Sugar Industry(ZSI), the need for developing improved varieties of sugarcane cannot be overemphasized. Since the establishment of experiment station in 1966, the Zimbabwe Sugar Industry (ZSI) has always been dependent on importation of crosses from other countries for development and release. However, the ZSI is now planning on developing its own crossing facilities. The ZSI gene bank has up to 746 imported varieties which can be used as potential parents in a breeding program. With the impending establishment of crossing facilities in the Zimbabwe sugar industry, morphological and molecular characterization of the available germplasm is essential to identify parental lines which can be used during crossing. The main objective of this study was to determine the genetic and phenotypic diversity of fifty ZSAES gene bank sugarcane accessions and assess how the existing diversity can be utilized in a breeding program in parental selection and variety identification. Fifty sugarcane...
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#3. RADX Sustains POT1 Function at Telomeres to Counteract RAD51 Binding, which Triggers Telomere Fragility
Joachim Lingner, Galina Glousker, Anna-Sophia Briod
The 3' terminal DNA extensions at chromosome ends can become engaged into multiple biochemical reactions during DNA replication, telomerase-mediated telomere extension, homology directed DNA repair, nucleolytic processing and DNA damage checkpoint activation. To keep these activities in check, telomeric 3' overhangs can be hidden in t-loop structures or they associate with specialized proteins such as POT1. Here, we explore the telomeric microenvironment using a proximity-dependent labeling approach and identify the oligonucleotide/oligosaccharide-binding (OB)-fold containing protein RADX. RADX binds single-stranded telomeric DNA throughout the cell cycle along with POT1, suppressing accumulation of fragile telomeres, which are indicative of telomere replication defects. Telomere fragility in POT1 and RADX double-depleted cells was due to accumulation of the RAD51 recombinase at telomeres. RADX also supports DNA damage signaling at POT1-depleted telomeres counteracting RAD51 binding. Thus, RADX represents next to POT1 a second...
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biorxivpreprint: RADX Sustains POT1 Function at Telomeres to Counteract RAD51 Binding, which Triggers Telomere Fragility https://t.co/fXMFkyajnz #bioRxiv
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#4. Human POT1 Prevents Severe Telomere Instability Induced by Homology Directed DNA Repair
Galina Glousker, Anna-Sophia Briod, Manfredo Quadroni, Joachim Lingner
The evolutionarily conserved POT1 protein binds single stranded G-rich telomeric DNA and has been implicated in contributing to telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through genetics and proteomics discovering that the complete absence of POT1 leads to severe telomere maintenance defects that had not been anticipated from previous depletion studies. Conditional deletion of POT1 in HEK293E cells gives rise to rapid telomere elongation and length heterogeneity, branched telomeric DNA structures, telomeric R-loops and telomere fragility. We determine the telomeric proteome upon POT1-loss implementing an improved telomeric chromatin isolation protocol. We identify a large set of proteins involved in nucleic acid metabolism that engage with telomeres upon POT1-loss. Inactivation of the homology directed repair machinery suppresses POT1-loss mediated telomeric DNA defects. Our results unravel as major function of human POT1 the suppression of telomere...
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Total Words: 17716
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