Top 2 Biorxiv Papers Today in Developmental Biology


1.997 Mikeys
#1. The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila
Henry Wilky, Sahla Syed, Joao Raimundo, Bomyi Lim, Amanda A Amodeo
Early embryos must rapidly generate large numbers of cells to form an organism. Many species accomplish this through a series of rapid, reductive, and transcriptionally silent cleavage divisions. Previous work has demonstrated that before both cell cycle elongation and zygotic genome activation (ZGA), the number of divisions is regulated by the ratio of nuclear content to cytoplasm (N/C). To understand how the N/C ratio affects the timing of ZGA we directly assayed the behavior of several previously identified N/C-ratio-dependent genes using the MS2-MCP reporter system in living Drosophila embryos with altered ploidy and cell cycle time. For every gene that we examined, we found that transcription is delayed in haploids. The N/C ratio influences transcription through two separate modes of action. For many genes the effect of ploidy can be entirely accounted for by changes in cell cycle duration. However, for a subset of genes the N/C ratio directly affects the probability of transcription initiation. While it appears that cell...
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biorxivpreprint: The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila https://t.co/SgCo4SK79j #bioRxiv
AAAmodeo: Decided to try something different for paper 3. Now on #bioRxiv ! We show that many transcripts respond to the N/C ratio indirectly via cell cycle, but frs (and others?) change activation probability independent of time = direct N/C ratio sensing! https://t.co/80smmRrgsn
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Authors: 5
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1.997 Mikeys
#2. Self-organised symmetry breaking in zebrafish reveals feedback from morphogenesis to pattern formation
Vikas Trivedi, Timothy Fulton, Andrea Attardi, Kerim Anlas, Chaitanya Dingare, Alfonso Martinez Arias, Benjamin J Steventon
A fundamental question in developmental biology is how the early embryo breaks initial symmetry to establish the spatial coordinate system later important for the organisation of the embryonic body plan. In zebrafish, this is thought to depend on the inheritance of maternal mRNAs [1 to 3], cortical rotation to generate a dorsal pole of beta-catenin activity [4 to 8] and the release of Nodal signals from the yolk syncytial layer (YSL) [9 to 12]. Recent work aggregating mouse embryonic stem cells has shown that symmetry breaking can occur in the absence of extra-embryonic tissue [19,20]. To test whether this is also true in zebrafish, we separated embryonic cells from the yolk and allowed them to develop as aggregates. These aggregates break symmetry autonomously to form elongated structures with an anterior-posterior pattern. Extensive cell mixing shows that any pre-existing asymmetry is lost prior to the breaking morphological symmetry, revealing that the maternal pre-pattern is not strictly required for early embryo patterning....
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Authors: 7
Total Words: 5203
Unqiue Words: 1869

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