Top 10 Biorxiv Papers Today in Developmental Biology


2.002 Mikeys
#1. Fetus-derived IGF2 matches placental development to fetal demand
Ionel Sandovici, Aikaterini Georgopoulou, Antonia S Hufnagel, Samira N Schiefer, Fatima Santos, Katharina Hoelle, Brian Y.H. Lam, Giles S.H. Yeo, Keith Burling, Jorge Lopez-Tello, Moritz Reiterer, Abigail L. Fowden, Graham J. Burton, Amanda N. Sferruzzi-Perri, Cristina M. Branco, Miguel Constancia
Growth of a fetus is dependent upon the functional capacity of its placenta, but how the latter is matched to fetal demands is currently unknown. Critically, there is continuous expansion of the feto-placental microvasculature throughout pregnancy, along with morphogenic modifications in the overlying trophoblast epithelium. Here we demonstrate, through fetal and trophoblast specific genetic manipulations in the mouse, that signalling by IGF2 from the feto-placental endothelium and endocrine actions of circulating fetal IGF2 are required. We provide evidence that endothelial and fetal-derived IGF2 plays an important role in trophoblast morphogenesis, acting through Gcm1 and Synb. The effects on placental microvasculature expansion are mediated through IGF2R and angiopoietin-Tie2/TEK signalling. Thus, our study reveals a direct role for IGF2-IGF2R axis on matching fetal demand to placental supply and establishes the principle that hormone-like signals from the fetus play important roles in the control of placental vascularization...
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ObsGynaeCam: A new preprint "Fetus-derived IGF2 matches placental development to fetal demand" from Ionel Sandovici, Miguel Constancia and colleagues @biorxivpreprint https://t.co/8GwvkJ4clj
ISandovici: @ISandovici Our work uncovers IGF2 as a hormone-like molecule that allows communication between the growing fetus and the placenta. https://t.co/MEuXaq4ty3
ISandovici1: Our work uncovering IGF2 as a hormone-like molecule that allows communication between the growing fetus and the placenta. https://t.co/cMj3XpprZQ
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Sample Sizes : [57, 46189, 7079, 67, 4, 60, 10, 22, 36, 34, 6, 4, 8, 13, 7, 57, 68, 6, 4, 68, 918, 5, 617, 326, 915, 920, 39, 49, 38, 34, 12, 9, 67, 711, 9, 1112]
Authors: 16
Total Words: 14702
Unqiue Words: 4963

2.0 Mikeys
#2. Phase separation during mouse early embryonic development and underlying genetic and epigenetic correlations
Hui Quan, Sirui Liu, Yu Zhang, Wei Xie, Yi Qin Gao
Chromatin undergoes drastic organization and epigenetic reprogramming during embryonic development in mammals. However, the relationship among global structural change, epigenetic reprogramming, and functional implementation is largely unknown. Based on the analysis of latest published Hi-C data of post-implantation stages, we present a consistent view of the chromatin structural change and the corresponding sequence dependence. Two types of sequentially, genetically and transcriptionally distinct domains, forests and prairies, show systematic and overall increase of spatial segregation during embryonic development, but with notable mixing occurring at two stages, ZGA and implantation. The segregation level change largely coincides with the change of genetic and epigenetic properties. Detailed gene functions in specific phase-changing domains during implantation were analyzed, based on which a possible mechanism of functional realization during implantation was proposed. Interestingly, body temperature changes coincide with the...
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amjeve: Phase separation during mouse early embryonic development and underlying genetic and epigenetic correlations https://t.co/Hjrgw4z8sC
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Sample Sizes : None.
Authors: 5
Total Words: 7419
Unqiue Words: 1888

1.999 Mikeys
#3. HMGB1 orchestrates uterine macrophage trafficking to safeguard embryo implantation
Shizu Aikawa, Wenbo Deng, Xiaohuan Liang, Jia Yuan, Amanda Bartos, Xiaofei Sun, SK Dey
A reciprocal communication between the implantation-competent blastocyst and the receptive uterus is essential to implantation. Blastocyst implantation is considered to be a regulated proinflammatory response in the uterus, however the underlining mechanism remains elusive. Here, we provide genetic evidence that High-mobility group protein Box-1 (HMGB1), expressed in uterine cell nuclei, restricts inflammatory responses during the periimplantation period. Conditional deletion of uterine Hmgb1 by using a Pgr-Cre driver (Pgrcre/+Hmgb1f/f) shows substantial infertility because of defective implantation and subsequent adverse ripple effects. These mice accumulate and retain an increased number of macrophages in the stroma on day 4 of pregnancy with a unique enrichment of macrophages in the stroma encircling the blastocyst on day 5, evoking inflammatory responses. These results are in contrast to previous findings that HMBG1 is an internal alarmin. In search for the mechanism, we found that Hmgb1-deleted stromal cells show reduced...
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dwb0211: HMGB1 orchestrates uterine macrophage trafficking to safeguard embryo implantation https://t.co/t1BfVD5tfm
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Sample Sizes : None.
Authors: 7
Total Words: 12503
Unqiue Words: 3848

1.998 Mikeys
#4. Cyto-architecture constrains a photoactivation induced tubulin gradient in the syncytial Drosophila embryo
Sameer Thukral, Bivash Kaity, Bipasha Dey, Swati Sharma, Amitabha Nandi, Mithun Mitra, Richa Rikhy
Drosophila embryogenesis begins with nuclear division in a common cytoplasm forming a syncytial cell. Morphogen gradient molecules spread across nucleo-cytoplasmic domains to pattern the body axis of the syncytial embryo. The diffusion of molecules across the syncytial nucleo-cytoplasmic domains is potentially constrained by association with the components of cellular architecture, however the extent of restriction has not been examined so far. Here we use photoactivation (PA) to generate a source of cytoplasmic or cytoskeletal molecules in order to monitor the kinetics of their spread in the syncytial Drosophila embryo. Photoactivated PA-GFP and PA-GFP-Tubulin within a fixed anterior area diffused along the antero-posterior axis. These molecules were enriched in cortical cytoplasm above the yolk-filled center suggesting that the cortical cytoplasm is phase separated from the yolk-filled center. The length scales of diffusion were extracted using exponential fits under steady state assumptions. PA-GFP spread to greater distance as...
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Sample Sizes : [3, 3, 3, 3, 6, 3, 3, 3, 84, 3, 44, 30, 3, 3, 3, 53, 25, 3, 3, 3, 63]
Authors: 7
Total Words: 12443
Unqiue Words: 3489

1.998 Mikeys
#5. KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control
Navroop K Dhaliwal, Luis E Abatti, Jennifer A Mitchell
Embryonic stem (ES) cells are regulated by a network of transcription factors which maintain the pluripotent state. Differentiation relies on downregulation of pluripotency transcription factors disrupting this network. While investigating transcriptional regulation of the pluripotency transcription factor Klf4, we observed homozygous deletion of distal enhancers caused 17 fold decrease in Klf4 transcript but surprisingly decreased protein levels by <2 fold indicating post-transcriptional control of KLF4 protein overrides transcriptional control. The lack of sensitivity of KLF4 to transcription is due to high protein stability (half-life of >24hr). This stability is context dependent and disrupted during differentiation, evidenced by a shift to a half-life of <2hr. KLF4 protein stability is maintained through interaction with other pluripotency transcription factors (NANOG, SOX2 and STAT3) that together facilitate association of KLF4 with RNA polymerase II. In addition, the KLF4 DNA binding and transactivation domains are required...
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biorxivpreprint: KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control https://t.co/UQPIP9S4NB #bioRxiv
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Sample Sizes : None.
Authors: 3
Total Words: 10088
Unqiue Words: 2015

1.996 Mikeys
#6. SWI/SNF component BAF250a coordinates OCT4 and WNT signaling pathway to control cardiac lineage differentiation
Ienglam Lei, Shuo Tian, Victor Chen, Yong Zhao, Zhong Wang
Dissecting epigenetic mechanisms controlling early cardiac differentiation will provide insights into heart regeneration and heart disease treatment. SWI/SNF complexes remodel nucleosomes to regulate gene expression and play a key role in organogenesis. Here we reported a unique function of BAF250a in regulating the physical interaction of OCT4 and β-CATENIN during cardiac lineage differentiation from human ESCs. BAF250a deletion greatly reduced the physical interaction between OCT4 and β-CATENIN but did not alter the expression of β-CATENIN and OCT4 in the mesodermal progenitor cells. BAF250a ablation led to decreased recruitment of OCT4 and β-CATENIN at promoters of key mesodermal lineage genes, such as MESP1 and EOMES. Subsequently, the expression of lineage specific genes was down-regulated whereas the expression of pluripotent genes was up-regulated. In parallel, BAF250a ablation also altered recruitments of OCT4 and β-CATENIN to the promoter of CCND2 and CCND3, two key genes for S phase entry during cell cycle. Consequently,...
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Sample Sizes : None.
Authors: 5
Total Words: 6013
Unqiue Words: 1724

1.996 Mikeys
#7. The Prrx1 limb enhancer marks an adult population of injury-responsive, multipotent dermal fibroblasts
Joshua D. Currie, Lidia Grosser, Prayag Murawala, Maritta Schuez, Martin Michel, Elly M. Tanaka, Tatiana Sandoval-Guzman
The heterogeneity of adult tissues has been posited to contribute toward the loss of regenerative potential in mammals. Here we characterize an adult population of dermal fibroblasts that maintain expression of a Prrx1 enhancer which originally marked mesenchymal limb progenitors. Prrx1 enhancer-positive cells (Prrx1enh+) make up a small subset of adult dermal cells (~0.2%) and reside mainly within specific dermal perivascular and hair follicle niches. Upon injury, however, Prrx1enh+ cells readily migrate into the wound bed and amplify on average 16-fold beyond their uninjured numbers. Additionally, Prrx1enh+ cells emigrate out of their dermal niches following wounding and contribute to subcutaneous tissue. Prrx1enh+ cells are uniquely injury-responsive and do not contribute to tissue homeostasis or enriched by neonatal-like Wnt signaling. Prrx1enh+ cells represent a potent regenerative cell population that, despite being a meager minority in adult skin, demonstrate the potential to tip the balance of mammalian wound healing...
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Sample Sizes : [6, 10, 6, 4]
Authors: 7
Total Words: 9380
Unqiue Words: 2546

0.0 Mikeys
#8. GFP-Forked, a genetic reporter for studying Drosophila oocyte polarity
Uri Abdu, Raju Baskar, Bakhrat Anya
The polarized organization of the Drosophila oocyte can be visualized by examining the asymmetric localization of mRNAs, which is supported by networks of polarized microtubules (MTs). In this study, we used the gene forked, the putative Drosophila homologue of espin, to develop a unique genetic reporter for asymmetric oocyte organization. We generated a null allele of the forked gene using the CRISPR-Cas9 system and found that forked is not required for determining the axes of the Drosophila embryo. However, ectopic expression of a truncated form of GFP-Forked generated a distinct network of asymmetric Forked, which first accumulated at the oocyte posterior and was then restricted to the anterolateral region of the oocyte cortex in mid-oogenesis. This localization pattern resembled that reported for the polarized MTs network. Indeed, pharmacological and genetic manipulation of the polarized organization of the oocyte showed that the filamentous Forked network diffused throughout the entire cortical surface of the oocyte, as would...
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biorxivpreprint: GFP-Forked, a genetic reporter for studying Drosophila oocyte polarity https://t.co/VIdsNDmCwv #bioRxiv
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Sample Sizes : [20, 40, 45, 20, 35, 20, 30]
Authors: 3
Total Words: 6254
Unqiue Words: 1675

0.0 Mikeys
#9. let-7 controls the transition to adulthood by releasing select transcriptional regulators from repression by LIN41
Florian Aeschimann, Anca Neagu, Magdalene Rausch, Helge Grosshans
Development of multicellular organisms relies on faithful temporal control of cell fates. In Caenorhabditis elegans, the heterochronic pathway governs temporal patterning of somatic cells. This function may be phylogenetically conserved as several heterochronic genes have mammalian orthologues, and as the heterochronic let-7 miRNA and its regulator LIN28 appear to time the onset of puberty in mice and humans. Here, we have investigated how let-7 promotes the transition to adulthood in C. elegans. We find that let-7 controls each of three relevant processes, namely male and female sexual organ morphogenesis as well as changes in skin progenitor cell fates, through the same single target, lin-41. LIN41 in turn silences two pairs of targets post-transcriptionally, by binding and silencing their mRNAs. The EGR-type transcription factor LIN-29a and its co-factor, the NAB1/2 orthologous MAB-10, mediate control of progenitor cell fates and vulval integrity. By contrast, male tail development depends on regulation of the DM...
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RNApreprints: Aeschimann et al: let-7 controls the transition to adulthood by releasing select transcriptional regulators from repression by LIN41 https://t.co/yQCIUVL4DK
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Sample Sizes : [20, 80, 20]
Authors: 4
Total Words: 16228
Unqiue Words: 3777

0.0 Mikeys
#10. Development of a robust automated tool for the annotation of embryo morphokinetic parameters
Magalie Feyeux, Arnaud REIGNIER, Maxime Mocaer, Jenna Lammers, Dimitri Meistermann, Sandrine Vandormael-Pournin, Michel Cohen-Tannoudji, Paul Barriere, Perrine Paul-Gilloteaux, Laurent David, Thomas Freour
Study question: Is it possible to automatically annotate human embryo development in time-lapse devices, with results comparable to manual annotation? Summary answer: We developed an automated tool for the annotation of embryo morphokinetic parameters having a high concordance with expert manual annotation in a large scale-study. What is known already: Morphokinetic parameters obtained with time-lapse devices are increasingly used for human embryo quality assessment. However, their annotation is time-consuming and can be operator-dependent, highlighting the need of developing automated approaches. Study design, size, duration: This monocentric pilot study was conducted using 701 blastocysts originating from 584 couples undergoing IVF with embryo culture in a time-lapse device and on 4 mouse embryos. Participants/materials, setting, methods: An automated annotation tool was developed based on grey level coefficient of variation and detection of the thickness of the zona pellucida. The timings of cellular events obtained with the...
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biorxivpreprint: Development of a robust automated tool for the annotation of embryo morphokinetic parameters https://t.co/6CRS7LrZsL #bioRxiv
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Sample Sizes : None.
Authors: 11
Total Words: 6063
Unqiue Words: 1982

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