Top 7 Biorxiv Papers Today in Developmental Biology


2.009 Mikeys
#1. Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation
Zubing Cao, Di Gao, Tengteng Xu, Ling Zhang, Xu Tong, Dandan Zhang, Yiqing Wang, Wei Ning, Xin Qi, Yangyang Ma, Kaiyuan Ji, Tong Yu, Yunsheng Li, Yunhai Zhang
Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both the cumulus cell and the oocyte via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these results demonstrate for the first...
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biorxivpreprint: Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation https://t.co/QHjLCcvjEu #bioRxiv
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Sample Sizes : None.
Authors: 14
Total Words: 12549
Unqiue Words: 3791

2.007 Mikeys
#2. Effects of short-term resistance training and tapering on maximal strength, peak power, throwing ball velocity, and sprint performance in handball players
Souhail Hermassi, Aloui Ghaith, René Schwesig, Roy J. Shephard, Mohamed Souhaiel Chelly
The purpose of this study was to assess the effect of short-term resistance training and two weeks of tapering on physical performances in handball players. Following a ten-week progressive resistance training program, subjects were divided between an experimental (n = 10) and a control group (n = 10). The experimental group completed a resistance training program, followed by a two-week period when the training intensity was tapered by 60%, while the control group maintained their typical pattern of training. Muscle power (force-velocity test and squat and counter-movement jump tests), sprinting ability (10m and 30m), ability to change direction (T-half test) and throwing velocity (a 3-step throw with a run, and a jump throw) were evaluated before training, at the end of training and after tapering. The experimental group showed significantly larger interaction effects for the 10-week training period (12/15, 80%), than for the following 2 weeks of tapering (10/15, 67%), with the largest gains being in 15 m sprint times (d=3.78)...
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biorxivpreprint: Effects of short-term resistance training and tapering on maximal strength, peak power, throwing ball velocity, and sprint performance in handball players https://t.co/FhWrERCeKe #bioRxiv
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Sample Sizes : [3, 4, 4, 4, 4, 10]
Authors: 5
Total Words: 7971
Unqiue Words: 2434

1.997 Mikeys
#3. Method to generate growth and degrowth models obtained from combinations of existing models applied to agrarian sciences
André Santos, Guilherme Rocha Moreira, Frank Gomes-Silva, Cícero Carlos Ramos de Brito, Maria Lindomárcia Costa, Luiz Gustavo Ribeiro Pereira, Rogério Martins Maurício, José Augusto Gomes de Azevêdo, José Marques Pereira, Alexandre Lima Ferreira, Moacyr Cunha Filho
Mathematical models that describe gas production are widely used to estimate the rumen degradation digestibility and kinetics. The present study presents a method to generate models by combining existing models and to propose the von Bertalanffy-Gompertz two-compartment model based on this method. The proposed model was compared with the logistic two-compartment one to indicate which best describes the kinetic curve of gas production through the semi-automated in vitro technique from different pinto peanut cultivars. The data came from an experiment grown and harvested at the Far South Animal Sciences station (Essul) in Itabela, BA, Brazil and gas production was read at 2, 4, 6, 8, 10, 12, 14, 17, 20, 24, 28, 32, 48, 72, and 96 h after the start of the in vitro fermentation process. The parameters were estimated by the least squares method using the iterative Gauss-Newton process in the software R version 3.4.1. The best model to describe gas accumulation was based on the adjusted coefficient of determination, residual mean...
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Authors: 11
Total Words: 5275
Unqiue Words: 1882

1.996 Mikeys
#4. Modular tissue-specific regulation of doublesex underpins sexually dimorphic development in Drosophila
Gavin Rice, Olga Barmina, David Luecke, Kevin Hu, Michelle Arbeitman, Artyom Kopp
The ability of a single genome to produce distinct and often dramatically different male and female forms is one of the wonders of animal development. In most animals, sex-specific phenotypes are shaped by interactions between a sex determination pathway and spatial patterning gene networks. In Drosophila melanogaster , most sexually dimorphic traits are controlled by sex-specific isoforms of the doublesex ( dsx ) transcription factor, and dsx expression is mostly limited to cells that give rise to sexually dimorphic traits. However, it is unknown how this mosaic of sex-naive and sex-aware tissues arises. Here, we characterize the cis-regulatory sequences that control dsx expression in the foreleg, which contains multiple types of sex-specific sensory organs. We find that separate modular enhancers are responsible for dsx expression in each sexually dimorphic organ. Expression of dsx in the sex comb is co-regulated by two enhancers with distinct spatial and temporal specificities that are separated by a genitalia-specific...
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Authors: 6
Total Words: 11959
Unqiue Words: 3410

1.992 Mikeys
#5. ERK1/2 signalling dynamics promote neural differentiation by regulating the polycomb repressive complex
Claudia I Semprich, Vicki Metzis, Harshil Patel, James Briscoe, Kate G. Storey
Fibroblast Growth Factor (FGF) is a neural inducer in many vertebrate embryos, but how it regulates chromatin organization to coordinate the activation of neural genes is unclear. Moreover, for differentiation to progress FGF signalling has to decline. Why this signalling dynamic is required has not been determined. Here we show that dephosphorylation of the FGF effector kinase ERK1/2 rapidly increases chromatin accessibility at neural genes in mouse embryos and, using ATAC-seq in human embryonic stem cell derived spinal cord precursors, we demonstrate that this occurs across hundreds of neural genes. Importantly, while Erk1/2 inhibition induces precocious neural gene transcription, this step involves dissociation of the polycomb repressive complex from gene loci and takes places independently of subsequent loss of the repressive histone mark H3K27me3 and transcriptional onset. We find that loss of ERK1/2 activity but not its occupancy at neural genes is critical for this mechanism. Moreover, transient ERK1/2 inhibition is...
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Sample Sizes : [3, 3, 3]
Authors: 5
Total Words: 19314
Unqiue Words: 5467

1.992 Mikeys
#6. Tamoxifen Suppresses Pancreatic β-Cell Proliferation in Mice
Surl-Hee Ahn, Anne Granger, Matthew M Rankin, Carol J Lam, Aaron R Cox, Jake A Kushner
Tamoxifen is a mixed agonist/antagonist estrogen analogue that is frequently used to induce conditional gene deletion in mice using Cre-loxP mediated gene recombination. Tamoxifen is routinely employed in extremely high-doses relative to typical human doses to induce efficient gene deletion in mice. Although tamoxifen has been widely assumed to have no influence upon β-cells, the acute developmental and functional consequences of high-dose tamoxifen upon glucose homeostasis and adult β-cells are largely unknown. We tested if tamoxifen influences glucose homeostasis in male mice of various genetic backgrounds. We then carried out detailed histomorphometry studies of mouse pancreata. We also performed gene expression studies with islets of tamoxifen-treated mice and controls. Tamoxifen had modest effects upon glucose homeostasis of mixed genetic background (F1 B6129SF1/J) mice, with fasting hyperglycemia and improved glucose tolerance but without overt effects on fed glucose levels or insulin sensitivity. Tamoxifen inhibited...
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Sample Sizes : [10, 10, 10, 10, 5]
Authors: 6
Total Words: 14025
Unqiue Words: 4632

1.991 Mikeys
#7. Transcriptomics supports that pleuropodia of insect embryos function in degradation of the serosal cuticle to enable hatching
Barbora Konopova, Elisa Buchberger, Alastair Crisp
Pleuropodia are limb-derived vesicular organs that transiently appear on the first abdominal segment of embryos from the majority of insect orders. They are missing in the model Drosophila and little is known about them. Experiments carried out on orthopteran insects eighty years ago indicated that the pleuropodia secrete a hatching enzyme that at the end of embryogenesis digests the serosal cuticle to enable the larva to hatch. This hypothesis contradicts the view that insect cuticle is digested by enzymes produced by the tissue that deposited it. We studied the development of the pleuropodia in embryos of the locust Schistocerca gregaria (Orthoptera) using transmission electron microscopy. RNA-seq was applied to generate a comprehensive embryonic reference transcriptome that was used to study genome-wide gene expression of ten stages of pleuropodia development. We show that the mature and secretion releasing pleuropodia are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes...
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Authors: 3
Total Words: 18484
Unqiue Words: 6154

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