Top 9 Biorxiv Papers Today in Cell Biology


2.0 Mikeys
#1. Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation
Andrew Keniry, Natasha Jansz, Linden J. Gearing, Iromi Wanigasuriya, Joseph Chen, Christian M Nefzger, Peter F Hickey, Quentin Gouil, Joy Liu, Kelsey Breslin, Megan Iminitoff, Tamara Beck, Andres Tapia del Fierro, Sarah A Kinkel, Phillippa C Taberlay, Tracy Willson, Miha Pakusch, Matthew E Ritchie, Douglas J Hilton, Jose Maria Polo, Marnie E Blewitt
Although female pluripotency significantly differs to male, complications with in vitro culture of female embryonic stem cells (ESC) have severely limited the use and study of these cells. We report a replenishable female ESC system, Xmas, that has enabled us to optimise a protocol for preserving the XX karyotype. Our protocol also improves male ESC fitness. We utilised our Xmas ESC system to screen for regulators of the female-specific process of X chromosome inactivation, revealing chromatin remodellers Smarcc1 and Smarca4 as key regulators of establishment of X inactivation. The remodellers create a nucleosome depleted region at gene promotors on the inactive X during exit from pluripotency, without which gene silencing fails. Our female ESC system provides a tractable model for XX ESC culture that will expedite study of female pluripotency and has enabled us to discover new features of the female-specific process of X inactivation.
more | pdf
Figures
None.
Tweets
biorxivpreprint: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation https://t.co/YfBLXx5vjT #bioRxiv
biorxiv_cellbio: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X ... https://t.co/Vi03mCL0F2 #biorxiv_cellbio
PromPreprint: Xmas ESC: A new female embryonic stem cell system that reveals the BAF complex as a key regulator of the establishment of X chromosome inactivation https://t.co/tA6IYOz00S
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 21
Total Words: 0
Unqiue Words: 0

2.0 Mikeys
#2. Proteostasis and Energetics as Proteome Hallmarks of Aging and Influenza Challenge in Pulmonary Disease
Salvatore Loguercio, Darren M Hutt, Alexander Rosa Campos, Thomas Stoeger, Rogan A Grant, Alexandra C McQuattie-Pimentel, Hiam Abdala-Valencia, Ziyan Lu, Nikita Joshi, Karen Ridge, Navdeep S Chandel, Jacob I Sznajder, Richard I Morimoto, Alexander V Misharin, G.R. Scott Budinger, William E Balch
Aging is associated with an increased risk for the development of many diseases. This is exemplified by the increased incidence of lung injury, muscle dysfunction and cognitive impairment in the elderly following influenza infection. Because the infectious cycle of flu is dependent upon the properties of the host, we examined the proteome of alveolar macrophages (AM) and type 2 cells (AT2) obtained from young (3 months) and old (18 months) na&iumlve mice and mice exposed to influenza A. Our proteomics data show that there is a maladaptive collapse of the proteostasis network (PN) and changes in mitochondrial pathways in the aged na&iumlve AM and AT2 proteomes. The mitochondrial imbalance and proteostatic collapse seen in aged cells places an excessive folding burden on these cells, which is further exacerbated following exposure to influenza A. Specifically, we see an imbalance in Hsp70 co-chaperones involved in protein folding and Hsp90 co-chaperones important for stress signaling pathways that are essential for cellular...
more | pdf
Figures
Tweets
Github

Current Proteostasis annotation for human proteins curated by the Balch lab

Repository: PPT_annotation
User: balchlab
Language: None
Stargazers: 0
Subscribers: 0
Forks: 0
Open Issues: 0
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 16
Total Words: 14775
Unqiue Words: 3526

1.999 Mikeys
#3. VE-cadherin endocytosis controls vascular integrity and patterning during development
Cynthia Grimsley Myers, Robin H Isaacson, Chantel M Cadwell, Jazmin Campos, Marina S Hernandes, Kenneth R Myers, Tadahiko Seo, William Giang, Kathy K Griendling, Andrew P Kowalczyk
Tissue morphogenesis requires dynamic intercellular contacts that are subsequently stabilized as tissues mature. The mechanisms governing these competing adhesive properties are not fully understood. Using gain- and loss-of-function approaches, we tested the role of p120-catenin (p120) and VE-cadherin (VE-cad) endocytosis in vascular development using mouse mutants that exhibit increased (VE-cadGGG/GGG) or decreased (VE-cadDEE/DEE) internalization. VE-cadGGG/GGG mutant mice exhibited reduced VE-cad-p120 binding, reduced VE-cad levels, microvascular hemorrhaging, and decreased survival. By contrast, VE-cadDEE/DEE mutants exhibited normal vascular permeability but displayed microvascular patterning defects. Interestingly, VE-cadDEE/DEE mutant mice did not require endothelial p120, demonstrating that p120 is dispensable in the context of a stabilized cadherin. In vitro , VE-cadDEE mutant cells displayed defects in polarization and cell migration that were rescued by uncoupling VE-cadDEE from actin. These results indicate that...
more | pdf
Figures
Tweets
biorxivpreprint: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/QonjRR86bh #bioRxiv
biorxiv_cellbio: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/BfwfWwre2W #biorxiv_cellbio
ThompsonLab: RT @biorxivpreprint: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/QonjRR86bh #bioRxiv
Huveneers_Lab: RT @biorxivpreprint: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/QonjRR86bh #bioRxiv
KwiatkowskiLab: RT @biorxivpreprint: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/QonjRR86bh #bioRxiv
xtalmountain: RT @biorxivpreprint: VE-cadherin endocytosis controls vascular integrity and patterning during development https://t.co/QonjRR86bh #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : [5, 6, 5]
Authors: 10
Total Words: 16659
Unqiue Words: 3921

1.997 Mikeys
#4. Folding latency of fluorescent proteins affects the mitochondrial localization of fusion protein
Sayaka Kashiwagi, Yoichiro Fujioka, Aya O Satoh, Aiko Yoshida, Mari Fujioka, Prabha Nepal, Atsuhi Tsuzuki, Ozorea Aoki, Sarad Paudel, Hitoshi Sasajima, Yusuke Ohba
The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we constructed a color pallet comprising different organelle markers and found that FFPs targeted to the mitochondria were mislocalized when fused to certain types of FPs. Such FPs included several variants of Aequorea victoria green FP (aqGFP) and a monomeric variant of the red FP. Because the FFPs that are mislocalized include FPs with faster maturing or folding mutations, the increase in the maturation rate is likely to prevent their expected localization. Indeed, when we reintroduced amino acid substitutions so that the FP sequences were equivalent to that of wild-type aqGFP, FFP localization to the mitochondria was significantly enhanced. Moreover, similar amino acid substitutions improved the localization of...
more | pdf
Figures
Tweets
biorxivpreprint: Folding latency of fluorescent proteins affects the mitochondrial localization of fusion protein https://t.co/4ebyUcQ2Li #bioRxiv
biorxiv_cellbio: Folding latency of fluorescent proteins affects the mitochondrial localization of fusion protein https://t.co/qYPTDj0YJI #biorxiv_cellbio
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 11
Total Words: 10886
Unqiue Words: 3274

1.997 Mikeys
#5. StemBond hydrogels optimise the mechanical microenvironment for embryonic stem cells
Celine Labouesse, Chibeza C. Agley, Bao Xiu Tan, Moritz Hofer, Alex Winkel, Giuliano G. Stirparo, Hannah T. Stuart, Christophe M Verstreken, William Mansfield, Paul Bertone, Kristian Franze, Jose C. R. Silva, Kevin J. Chalut
Studies of mechanical signalling are typically performed by comparing cells cultured on soft and stiff hydrogel-based substrates. However, it is challenging to independently and robustly control both substrate stiffness and tethering of extracellular matrix (ECM) to substrates, making ECM tethering a potentially confounding variable in mechanical signalling investigations. Moreover, poor ECM tethering can lead to weak cell attachment. To address this, we developed StemBond hydrogels, a hydrogel formulation in which ECM tethering is stable and can be varied independently of stiffness. We show that soft StemBond hydrogels provide an optimal format for culturing embryonic stem (ES) cells. We find that soft StemBond substrates improve the homogeneity of ES cell populations, boost their self-renewal, and increase the efficiency of cellular reprogramming. Our findings underline how soft microenvironments impact mechanosensitive signalling pathways regulating self-renewal and differentiation, indicating that optimising the complete...
more | pdf
Figures
Tweets
biorxivpreprint: StemBond hydrogels optimise the mechanical microenvironment for embryonic stem cells https://t.co/4lGulwkvkE #bioRxiv
biorxiv_cellbio: StemBond hydrogels optimise the mechanical microenvironment for embryonic stem cells https://t.co/jdKPJ0epM2 #biorxiv_cellbio
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 13
Total Words: 18430
Unqiue Words: 4916

1.997 Mikeys
#6. Screening the Medicines for Malaria Venture Pathogen Box for invasion and egress inhibitors of the blood stage of Plasmodium falciparum reveals several inhibitory compounds
Madeline Grace Dans, Greta E Weiss, Danny W Wilson, Brad E Sleebs, Brendan Crabb, Tania F de Koning-Ward, Paul R Gilson
To identify potential inhibitors of egress and invasion in the asexual blood stage of Plasmodium falciparum , we screened the Medicines for Malaria Venture (MMV) Pathogen Box. This compound library comprises of 400 drugs against neglected tropical diseases, including 125 with antimalarial activity. For this screen, we utilised transgenic parasites expressing a bioluminescent reporter, Nanoluciferase (Nluc), to measure inhibition of parasite egress and invasion in the presence of the Pathogen Box compounds. At a concentration of 2 µM, we found 15 compounds that inhibited parasite egress by >40% and 24 invasion-specific compounds that inhibited invasion by >90%. We further characterised 11 of these inhibitors through cell-based assays and live cell microscopy and found two compounds that inhibited merozoite maturation in schizonts, one compound that inhibited merozoite egress, one compound that directly inhibited parasite invasion and one compound that slowed down invasion and arrested ring formation. The remaining compounds were...
more | pdf
Figures
Tweets
biorxivpreprint: Screening the Medicines for Malaria Venture Pathogen Box for invasion and egress inhibitors of the blood stage of Plasmodium falciparum reveals several inhibitory compounds https://t.co/CQxWaPrZzx #bioRxiv
biorxiv_cellbio: Screening the Medicines for Malaria Venture Pathogen Box for invasion and egress inhibitors of the blood stage of Plasmodium ... https://t.co/JDWakvIwFp #biorxiv_cellbio
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 7
Total Words: 18067
Unqiue Words: 4798

1.997 Mikeys
#7. Real-time dynamics of Plasmodium NDC80 as a marker for the kinetochore during atypical mitosis and meiosis
Rajan Pandey, Mohammad Zeeshan, David J.P. Ferguson, Robert Markus, Declan Brady, Emilie Daniel, Rebecca R. Stanway, Anthony A Holder, David S. Guttery, Rita Tewari
Eukaryotic cell proliferation requires chromosome replication and precise segregation, followed by cell division, to ensure daughter cells have identical genomic copies. The kinetochore is a multi-protein structure assembled on chromosome centromeres, which mediates attachment to spindle microtubules and chromosome segregation during mitosis. Plasmodium spp., the causative agent of malaria, undergoes closed mitosis with an intact nuclear membrane and intranuclear mitotic spindle. However, the regulation of the main events of mitosis and meiosis, including chromosome segregation, is poorly understood in this parasite. In part this is due to a distinct lack of markers for tracking kinetochore dynamics. Since NDC80 is the conserved component of the kinetochore complex, we labelled NDC80 with either a C-terminal GFP or mCherry tag by recombination at the endogenous locus to generate transgenic lines of the rodent malaria parasite Plasmodium berghei. We show that NDC80 is located at a single cluster of all kinetochores and examine its...
more | pdf
Figures
None.
Tweets
biorxivpreprint: Real-time dynamics of Plasmodium NDC80 as a marker for the kinetochore during atypical mitosis and meiosis https://t.co/L73QIxdpTK #bioRxiv
biorxiv_cellbio: Real-time dynamics of Plasmodium NDC80 as a marker for the kinetochore during atypical mitosis and meiosis https://t.co/wo4LF2Fg6f #biorxiv_cellbio
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 10
Total Words: 0
Unqiue Words: 0

1.997 Mikeys
#8. The ATG5 Interactome Links Clathrin Vesicular Trafficking With The ATG8 Lipidation Machinery For Autophagosome Assembly
Jon D Lane, Kiren Baines
Autophagosome formation involves the sequential actions of conserved ATG family proteins that regulate the lipidation of the ubiquitin-like modifier ATG8 at the nascent isolation membrane. Although the molecular steps driving this process are well understood, the source of membranes supplied for the expanding autophagosome and their mode of delivery remain uncertain. Here, we have used quantitative SILAC-based proteomics to identify proteins that associate with the ATG12~ATG5 conjugate that is crucial for ATG8 lipidation. Our datasets reveal a strong enrichment of regulators of clathrin-mediated vesicular trafficking, including clathrin heavy and light chains, and several clathrin adaptors. Also identified were PIK3C2A (a phosphoinositide 3-kinase involved in clathrin-mediated endocytosis) and HIP1R (a component of clathrin vesicles), and the absence of either of these proteins caused defects in autophagic flux in cell-based starvation assays. To determine whether the ATG12~ATG5 conjugate reciprocally influences trafficking within...
more | pdf
Figures
None.
Tweets
biorxivpreprint: The ATG5 Interactome Links Clathrin Vesicular Trafficking With The ATG8 Lipidation Machinery For Autophagosome Assembly https://t.co/dcBSX6Pu6m #bioRxiv
biorxiv_cellbio: The ATG5 Interactome Links Clathrin Vesicular Trafficking With The ATG8 Lipidation Machinery For Autophagosome Assembly https://t.co/8sr6E9tuzX #biorxiv_cellbio
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 2
Total Words: 0
Unqiue Words: 0

1.997 Mikeys
#9. Complexities of Microtubule Population Dynamics within the Mitotic Spindle
Aaron R Tipton, Gary J Gorbsky
The mitotic spindle functions to move chromosomes to alignment at metaphase, then segregate sister chromatids during anaphase. Analysis of spindle microtubule kinetics utilizing fluorescence dissipation after photoactivation described two main populations, a slow and a fast turnover population, historically taken to reflect kinetochore versus non-kinetochore microtubules respectively. This two component demarcation seems likely oversimplified. Microtubule turnover may vary among different spindle microtubules, regulated by spatial distribution and interactions with other microtubules and with organelles such as kinetochores, chromosome arms, and the cell cortex. How turnover among various spindle microtubules is differentially regulated and its significance remains unclear. We tested the concept of kinetochore versus non-kinetochore microtubules by disrupting kinetochores through depletion of the Ndc80 complex. In the absence of functional kinetochores, microtubule dynamics still exhibited slow and fast turnover populations,...
more | pdf
Figures
None.
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 2
Total Words: 0
Unqiue Words: 0

About

Assert is a website where the best academic papers on arXiv (computer science, math, physics), bioRxiv (biology), BITSS (reproducibility), EarthArXiv (earth science), engrXiv (engineering), LawArXiv (law), PsyArXiv (psychology), SocArXiv (social science), and SportRxiv (sport research) bubble to the top each day.

Papers are scored (in real-time) based on how verifiable they are (as determined by their Github repos) and how interesting they are (based on Twitter).

To see top papers, follow us on twitter @assertpub_ (arXiv), @assert_pub (bioRxiv), and @assertpub_dev (everything else).

To see beautiful figures extracted from papers, follow us on Instagram.

Tracking 189,566 papers.

Search
Sort results based on if they are interesting or reproducible.
Interesting
Reproducible
Feedback
Online
Stats
Tracking 189,566 papers.