Top 8 Biorxiv Papers Today in Cell Biology


2.023 Mikeys
#1. GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution
Sine Yaganoglu, Nordine Helassa, Benjamin M Gaub, Maaike Welling, Jian Shi, Daniel J Müller, Katalin Török, Periklis Pantazis
Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. Here, we developed GenEPi, a genetically encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. We show that GenEPi has high specificity and spatiotemporal resolution for Piezo1-dependent mechanical stimuli, exemplified by resolving repetitive mechanical stimuli of spontaneously contracting cardiomyocytes within microtissues, in a non-invasive manner.
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biorxivpreprint: GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution https://t.co/h0qe7Y0sQ6 #bioRxiv
biorxiv_cellbio: GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution https://t.co/Y9BydpCw2h #biorxiv_cellbio
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Sample Sizes : [12, 13, 15, 19, 21, 21, 16, 27, 13, 18, 12, 15, 16, 19, 14, 17, 19, 21, 45, 13, 11, 21, 27, 16, 15]
Authors: 8
Total Words: 9737
Unqiue Words: 3513

2.012 Mikeys
#2. Ins2 gene bursting activity defines a mature beta-cell state
Honey Modi, Sis Skovso, Cara Ellis, Nicole A.J. Krentz, Yiwei B Zhao, Haoning Cen, Nilou Noursadeghi, Evgeniy Panzhinskiy, Xiaoke Hu, Derek A Dionne, Shouhong Xuan, Mark O Huisind, Timothy J Kieffer, Francis C Lynn, James Johnson
Heterogeneity within specific cell types is common and increasingly apparent with the advent of single-cell transcriptomics. Transcriptional and functional cellular specialization has been described for insulin-secreting beta-cells of the endocrine pancreas, including so-called extreme beta-cells exhibiting more than 2 fold higher insulin gene activity. However, it is not yet clear whether beta-cell heterogeneity is stable or reflects dynamic cellular states. We investigated the temporal kinetics of endogenous insulin gene activity using live-cell imaging, with complementary experiments employing FACS and single-cell RNA sequencing, in beta-cells from Ins2GFP knock-in mice. In vivo staining and FACS analysis of islets from Ins2GFP mice confirmed that at a given moment, around 25 percent of beta-cells exhibited significantly higher activity at the conserved insulin gene Ins2(GFP)HIGH. Live-cell imaging captured on and off bursting behaviour in single beta-cells that lasted hours to days. Single cell RNA sequencing determined that...
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biorxivpreprint: Ins2 gene bursting activity defines a mature beta-cell state https://t.co/EakiwUhRLQ #bioRxiv
biorxiv_cellbio: Ins2 gene bursting activity defines a mature beta-cell state https://t.co/dhzONyzELB #biorxiv_cellbio
sbotlite: RT @biorxivpreprint: Ins2 gene bursting activity defines a mature beta-cell state https://t.co/EakiwUhRLQ #bioRxiv
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Sample Sizes : [3, 3]
Authors: 15
Total Words: 9064
Unqiue Words: 2961

2.01 Mikeys
#3. Cryopreservation of human pluripotent stem cell-derived cardiomyocytes is not detrimental to their molecular and functional properties
Lettine van den Brink, Karina O. Brandao, Catarina Grandela, Mervyn P.H. Mol, Christine L. Mummery, Arie O. Verkerk, Richard P. Davis
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a powerful platform for in vitro modelling of cardiac diseases, safety pharmacology, and drug screening. All these applications require large quantities of well-characterised and standardised batches of hiPSC-CMs. Cryopreservation of hiPSC-CMs without affecting their biochemical or biophysical phenotype is essential for facilitating this, but ideally requires the cells being unchanged by the freeze-thaw procedure. We therefore compared the in vitro functional and molecular characteristics of fresh and cryopreserved hiPSC-CMs generated from two independent hiPSC lines. While the frozen hiPSC-CMs exhibited poorer replating than their freshly-derived counterparts, there was no difference in the proportion of cardiomyocytes retrieved from the mixed population when this was factored in. Interestingly, cryopreserved hiPSC-CMs from one line exhibited longer action potential durations. These results provide evidence that cryopreservation does not...
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biorxivpreprint: Cryopreservation of human pluripotent stem cell-derived cardiomyocytes is not detrimental to their molecular and functional properties https://t.co/5vYQOabRMK #bioRxiv
biorxiv_cellbio: Cryopreservation of human pluripotent stem cell-derived cardiomyocytes is not detrimental to their molecular and functional properties https://t.co/ygWFQUlcdq #biorxiv_cellbio
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Sample Sizes : None.
Authors: 7
Total Words: 0
Unqiue Words: 0

2.01 Mikeys
#4. Modulation of Actin network and Tau phosphorylation by HDAC6 ZnF UBP domain
Subashchandrabose Chinnathambi, Abhishek Ankur Balmik, Shweta Kishor Sonawane
Microtubule-associated protein Tau undergoes aggregation in Alzheimer's disease and a group of other related diseases collectively known as Tauopathies. In AD, Tau forms aggregates, which are deposited intracellularly as neurofibrillary tangles. HDAC6 plays an important role in aggresome formation where it recruits polyubiquitinated aggregates to the motor protein dynein. Here, we have studied the effect of HDAC6 ZnF UBP on Tau phosphorylation, ApoE localization, GSK-3β regulation and cytoskeletal organization in neuronal cells by immunocytochemistry. Immunocytochemistry reveals that HDAC6 ZnF UBP can modulate Tau phosphorylation and actin cytoskeleton organization when the cells are exposed to the domain. HDAC6 ZnF UBP treatment to cells does not affect their viability and resulted in enhanced neurite extension and formation of structures similar to podosomes, lamellipodia and podonuts suggesting its role in actin re-organization. Also, HDAC6 treatment showed increased nuclear localization of ApoE and tubulin localization in...
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biorxivpreprint: Modulation of Actin network and Tau phosphorylation by HDAC6 ZnF UBP domain https://t.co/vRP53rG56X #bioRxiv
biorxiv_cellbio: Modulation of Actin network and Tau phosphorylation by HDAC6 ZnF UBP domain https://t.co/4vxU83gtqi #biorxiv_cellbio
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Authors: 3
Total Words: 0
Unqiue Words: 0

2.001 Mikeys
#5. Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag
ESTHER BRASELMANN, Timothy J Stasevich, Kenneth Lyon, Robert T Batey, AMY E PALMER
Labeling and tracking biomolecules with fluorescent probes on the single molecule level enables quantitative insights into their dynamics in living cells. We previously developed Riboglow, a platform to label RNAs in live mammalian cells, consisting of a short RNA tag and a small organic probe that increases fluorescence upon binding RNA. Here, we demonstrate that Riboglow is capable of detecting and tracking single RNA molecules. We benchmark RNA tracking by comparing results with the established MS2 RNA tagging system. To demonstrate versatility of Riboglow, we assay translation on the single molecule level, where the translated mRNA is tagged with Riboglow and the nascent polypeptide is labeled with a fluorescent antibody. The growing effort to investigate RNA biology on the single molecule level requires sophisticated and diverse fluorescent probes for multiplexed, multi-color labeling of biomolecules of interest, and we present Riboglow as a new member in this toolbox.
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hauryliuk: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
TJesse62: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
Parikki: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
Juli_Bla: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
petry594: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
YangWeibing: RT @biorxiv_cellbio: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/MyTzJRzHwd #b…
AGuleren: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
lacks22: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
poppingjun: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
VWosika: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
HandeKarahan_: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
DoubleAsh3: RT @biorxivpreprint: Detection and quantification of single mRNA dynamics with the Riboglow fluorescent RNA tag https://t.co/aiu9j2uORC #b…
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Authors: 5
Total Words: 0
Unqiue Words: 0

1.998 Mikeys
#6. Spinocerebellar Ataxia Type 1 protein Ataxin-1 is signalled to DNA damage by Ataxia Telangiectasia Mutated kinase
Celeste E Suart, Alma M Perez, Ismael Al-Ramahi, Tamara Maiuri, Juan Botas, Ray Truant
Spinocerebellar ataxia type 1 and Huntington's disease are two CAG DNA expansion neurodegenerative diseases that have common disease modifier genes in the DNA damage response (DDR) pathway. We demonstrate that ataxin-1 localizes to sites of DNA damage in an ataxia telangiectasia mutated (ATM) kinase dependent manner and have identified the ATM substrate site in ataxin-1, thus defining a common mechanistic pathway in DDR between three age-onset neurodegenerative diseases.
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biorxivpreprint: Spinocerebellar Ataxia Type 1 protein Ataxin-1 is signalled to DNA damage by Ataxia Telangiectasia Mutated kinase https://t.co/CTdzivf8mg #bioRxiv
biorxiv_cellbio: Spinocerebellar Ataxia Type 1 protein Ataxin-1 is signalled to DNA damage by Ataxia Telangiectasia Mutated kinase https://t.co/91NQ2eka07 #biorxiv_cellbio
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Sample Sizes : None.
Authors: 6
Total Words: 5540
Unqiue Words: 1865

1.997 Mikeys
#7. Transfer of Septin Rings to Cytokinetic Remnants Directs Age-Sensitive ER stress Surveillance Cell Cycle Re-entry
Jesse T Chao, Francisco Pina, Masayuki Onishi, Yifat Cohen, Maya Schuldiner, Maho Niwa
During cell division, cell′s must actively pass on organelles. Previously, we discovered the endoplasmic reticulum (ER) stress surveillance (ERSU) pathway that ensures the inheritance of functional ER. Activation of the ERSU causes the septin ring to mislocalize, which blocks ER inheritance and cytokinesis. Here, we found that the septin ring mislocalizes to previously utilized cell division sites called cytokinetic remnants (CRMs). The transfer of the septin ring to CRMs requires Nba1, a negative polarity component that normally prevents septin ring formation at CRMs. Furthermore, septin ring movement to CRMs relies on the ERSU component Slt2, which is recruited by binding Bem1. During ER stress, Bem1 also binds the GTP exchange factor Cdc24, without activating Cdc42, a GTPase that normally establishes polarized growth. Failure to translocate septin rings to CRMs delays the cells ability to re-enter cell division when ER homeostasis is re-established. Thus, ER stress considers the history of previous cell cycle for future cell...
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Sample Sizes : None.
Authors: 6
Total Words: 18611
Unqiue Words: 3609

1.996 Mikeys
#8. Cloning human dental pulp cells and studying inter-clone diversity
Linna Guo, Ziang Zou, Ming Yan, Marcus Freytag, Reinhard E Friedrich, Lan Kluwe
Heterogeneity within a putative stem cell population presents a challenge for studies and applications of such cells. Cloning may provide a strategy for reducing heterogeneity. However, previous studies have the weakness in reliability of single-cell-origin of the colonies. The present study aims to apply an alternative method to obtain clonal dental pulp cells with increased reliability of single cell origin. Dental pulp cells were cultured from 13 human wisdom teeth. Primary cultures of 3 human benign tumors were included as comparison. Cells were seeded into wells of a 96-plate at a mean density of 1 cell/well. On the next day, wells were inspected one by one to identify wells with single cells which were followed for 3 weeks. Survived clones were expanded and further characterized. Single cells were observed in all cases, the number of single-cell-wells varied from 16 to 33. Three weeks later, survived and grown clonal cells were observed in 10 to 29 wells, giving surviving rates of 33-91%. By contrast, though single tumor...
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aviseka: RT @biorxiv_cellbio: Cloning human dental pulp cells and studying inter-clone diversity https://t.co/pQI9yJwgdh #biorxiv_cellbio
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Authors: 6
Total Words: 0
Unqiue Words: 0

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