Top 4 Biorxiv Papers Today in Bioengineering


0.0 Mikeys
#1. A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair
Kohei Nishimura, Ryotaro Yamada, Shinya Hagihara, Rie Iwasaki, Naoyuki Uchida, Takumi Kamura, Koji Takahashi, Keiko U Torii, Tatsuo Fukagawa
Auxin-Inducible Degron (AID) technology enables conditional depletion of targeted proteins. However, the applicability of the AID in vertebrate cells has been limited due to cytotoxicity caused by high auxin concentrations. Here, we establish an improved AID system using an engineered orthogonal auxin-TIR1 pair, which exhibits over 1,000 times stronger binding. With ~1,000-fold less auxin concentration, we achieved to generate the AID-based knockout cells in various human and mouse cell lines in a single transfection.
more | pdf
Figures
Tweets
biorxivpreprint: A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair https://t.co/LViJ9AopSk #bioRxiv
PlantoPhagy: RT @biorxivpreprint: A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair https://t.co/LViJ9AopSk #bioRxiv
jacquimonaghan: RT @biorxivpreprint: A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair https://t.co/LViJ9AopSk #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 9
Total Words: 4895
Unqiue Words: 1439

0.0 Mikeys
#2. Ultrasound imaging links soleus muscle neuromechanics and energetics during human walking with elastic ankle exoskeletons
Richard W Nuckols, Taylor J.M. Dick, Owen N. Beck, Gregory S. Sawicki
Unpowered exoskeletons with springs in parallel to human plantar flexor muscle-tendons can reduce the metabolic cost of walking. We used ultrasound imaging to look 'under the skin' and measure how exoskeleton stiffness alters soleus muscle contractile dynamics and shapes the user's metabolic rate during walking. Eleven participants (4F, 7M; age: 27.7 ± 3.3 years) walked on a treadmill at 1.25 m s-1 and 0% grade with elastic ankle exoskeletons (rotational stiffness: 0-250 Nm rad-1) in one training and two testing days. Metabolic savings were maximized (4.2%) at a stiffness of 50 Nm rad-1. As exoskeleton stiffness increased, the soleus muscle operated at longer lengths and improved economy (force/activation) during early stance, but this benefit was offset by faster shortening velocity and poorer economy in late stance. Changes in soleus activation rate correlated with changes in users' metabolic rate (p = 0.038, R2 = 0.44), highlighting a crucial link between muscle neuromechanics and exoskeleton performance; perhaps informing...
more | pdf
Figures
Tweets
biorxivpreprint: Ultrasound imaging links soleus muscle neuromechanics and energetics during human walking with elastic ankle exoskeletons https://t.co/B8qQcOHbBz #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : [11, 10, 10, 11]
Authors: 4
Total Words: 13513
Unqiue Words: 4186

0.0 Mikeys
#3. Control tools to selectively produce purple bacteria for microbial protein in raceway reactors
Abbas Alloul, Marta Cerruti, Damian Adamczyka, David Gregory Weissbrodt, Siegfried E. Vlaeminck
Purple non-sulfur bacteria (PNSB) show potential for microbial protein production on wastewater as animal feed. They offer good selectivity (i.e. uneven community with high abundance of one species) when grown anaerobically in the light. However, the cost of a closed anaerobic photobioreactor (PBR) is prohibitive for protein production. While open raceway reactors are cheaper, their feasibility to selectively grow PNSB is thus far unexplored. This study developed tools to boost PNSB abundance in the biomass of a raceway reactor fed with volatile fatty acids as carbon source. For oxygen availability as tool, not stirring in the night (i.e. reduced oxygen supply) elevated the PNSB abundance from 8% to 20%. For light availability as tool, a 24-h illumination increased the PNSB abundance from 8% to 31% compared to a 12-h light/12-h dark regime. A reactor run at 2-d sludge retention time at the highest surface-to-volume ratio (10 m2 m-3 increased light availability) showed productivities up to 0.2 g protein L-1 d-1 and the highest...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 5
Total Words: 9052
Unqiue Words: 2818

0.0 Mikeys
#4. What causes different coronal curve patterns in idiopathic scoliosis?
Saba Pasha
Background Adolescent idiopathic scoliosis (AIS) is a three-dimensional deformity of the spinal column in otherwise healthy adolescents. The underlying mechanisms associated with the spinal deformity development have been explored which delineated the role of the sagittal curvature of the spine. The patterns of the spinal deformity vary between the AIS patients as shown in several classification systems. It remains to further investigate how variations in sagittal profiles result in different coronal plane deformities in AIS and how these deformation patterns are intrinsically different. Methods A total of 71 Lenke 1 and 52 Lenke 5 AIS patients were included retrospectively. The 3D models of the spine were generated from biplanar radiographs to calculate the clinical spinal parameters, vertebral axial rotations, and the 3D centerline of the spinal curvature. A classification based on the centerlines' axial plane projection was developed. The 3D curvature of the centerline was calculated at each point. A 2D elastic rod finite...
more | pdf
Figures
Tweets
biorxivpreprint: What causes different coronal curve patterns in idiopathic scoliosis? https://t.co/tQNa5WBjuR #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : [39, 32, 52]
Authors: 1
Total Words: 5696
Unqiue Words: 1525

About

Assert is a website where the best academic papers on arXiv (computer science, math, physics), bioRxiv (biology), BITSS (reproducibility), EarthArXiv (earth science), engrXiv (engineering), LawArXiv (law), PsyArXiv (psychology), SocArXiv (social science), and SportRxiv (sport research) bubble to the top each day.

Papers are scored (in real-time) based on how verifiable they are (as determined by their Github repos) and how interesting they are (based on Twitter).

To see top papers, follow us on twitter @assertpub_ (arXiv), @assert_pub (bioRxiv), and @assertpub_dev (everything else).

To see beautiful figures extracted from papers, follow us on Instagram.

Tracking 257,111 papers.

Search
Sort results based on if they are interesting or reproducible.
Interesting
Reproducible
Feedback
Online
Stats
Tracking 257,111 papers.