Top 9 Biorxiv Papers Today in Bioengineering


2.017 Mikeys
#1. Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
Brian Robert Patton, Graeme E Johnstone, Gemma S Cairns
Particles of diamond in the 5 to 100 nm size range, known as nanodiamond, have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but they allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of nanodiamond can also be of use when characterising adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.
more | pdf
Figures
None.
Tweets
biorxivpreprint: Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy https://t.co/Oach0dUPk0 #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 3
Total Words: 6871
Unqiue Words: 2484

1.997 Mikeys
#2. Implementation of Design of Experiments (DOE) for Optimization of Feeding Strategy and Glyco-Engineering of Trastuzumab Biosimilar
Rasoul Mahboudi, Sepideh Samavat, Amir Afrah, Mehdi Khorshidtalab, Arezou Fadaei Tehran, Paria Motahari, Farnoush Jafari, Shayan Maleknia
Fed-batch cell culture is the most commonly used process for antibody production in biopharmaceutical industries. Basal media, feed, feeding strategy and glycan structures are always among the most important concerns during process development and optimization. In this study, first, a traditional screening study was performed to identify the top media/feed combinations by evaluating the cell culture performance including cell growth and protein titre. Optimization of the process was also performed using response surface methodology in order to find the most optimum feeding strategy and glucose set point regarding final titre of the recombinant monoclonal antibody being produced in Chinese hamster ovary cell line. The focus of this study is not only on titre, but also on product quality and comparability especially protein glycosylation. The prediction model of product titre as a function of feeding percentage and glucose set point was successfully applied for the second set of experiments that was performed for glycan improvement....
more | pdf
Figures
Tweets
biorxivpreprint: Implementation of Design of Experiments (DOE) for Optimization of Feeding Strategy and Glyco-Engineering of Trastuzumab Biosimilar https://t.co/JjswegpFAj #bioRxiv
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 8
Total Words: 10174
Unqiue Words: 3060

1.996 Mikeys
#3. A Truly Injectable Neural Stimulation Electrode Made From an In-Body Curing Polymer/Metal Composite
James K. Trevathan, Ian Baumgart, Evan N. Nicolai, Brian A. Gosink, Anders J. Asp, Megan L. Settell, Shyam R. Polaconda, Kevin Malerick, Sarah K. Brodnick, Weifeng Zeng, Bruce E. Knudsen, Andrea L. McConico, Zachary Sanger, Jannifer H. Lee, Johnathan M. Aho, Aaron J. Suminski, Erika K. Ross, J. Luis Lujan, Douglas J. Weber, Justin C. Williams, Manfred Franke, Kip A. Ludwig, Andrew J. Shoffstall
Implanted neural stimulation and recording devices hold vast potential to treat a variety of neurological conditions, but the invasiveness, complexity, and cost of the implantation procedure greatly reduce access to an otherwise promising therapeutic approach. To address this need, we have developed a novel electrode that begins as an uncured pre-polymer that is liquid and can be injected around a neuroanatomical target to minimize surgical manipulation. Referred to as the Injectrode, the electrode conforms to complex target structures such as a foramen or plexus, and within minutes cures in vivo forming an electrically conductive interface. The resulting interface has a Young's Modulus of under 100 kPa, which is orders of magnitude less stiff than conventional electrodes. To validate the Injectrode, we performed detailed electrochemical and microscopy characterization of material properties and validated the feasibility of using it to electrically stimulate the nervous system in pre-clinical animal models. Electrochemical testing...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : [5, 2]
Authors: 23
Total Words: 11044
Unqiue Words: 3031

1.996 Mikeys
#4. WAT's up!? - Organ-on-a-chip integrating human mature white adipose tissues for mechanistic research and pharmaceutical applications
Julia Rogal, Carina Binder, Elena Kromidas, Christopher Probst, Stefan Schneider, Katja Schenke-Layland, Peter Loskill
Obesity and its numerous adverse health consequences have taken on global, pandemic proportions. White adipose tissue (WAT) - a key contributor in many metabolic diseases - contributes about one fourth of a healthy human's body mass. Despite its significance, many WAT-related pathophysiogical mechanisms in humans are still not understood, largely due to the reliance on non-human animal models. In recent years, Organ-on-a-chip (OoC) platforms have developed into promising alternatives for animal models; these systems integrate engineered human tissues into physiological microenvironment supplied by a vasculature-like microfluidic perfusion. Here, we report the development of a novel OoC that integrates functional mature human WAT. The WAT-on-a-chip is a multilayer device that features tissue chambers tailored specifically for the maintenance of 3D tissues based on human primary adipocytes, with supporting nourishment provided through perfused media channels. The platform's capability to maintain long-term viability and...
more | pdf
Figures
None.
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 7
Total Words: 0
Unqiue Words: 0

1.996 Mikeys
#5. Laser Recording of Subcellular Neuron Activities
Yu-Cheng Chen, Xuzhou Li, Hongbo Zhu, Wei-Hung Weng, Xiaotian Tan, Qiushu Chen, Xueding Wang, Xudong Fan
Advances in imaging and recording of neural activities with a single neuron resolution have played a significant role in understanding neurological diseases in the past decade. Conventional methods relying on patch-clamp and electrodes are regarded as invasive, whereas fluorescence-based imaging tools are useful but still suffer from a low signal-to-noise ratio and low sensitivity. Here we developed a novel optical imaging and recording system by employing laser emissions to record the action potentials in single neurons and neuronal networks caused by subtle transients (Ca2+ concentration) in primary neurons in vitro with a subcellular and single-spike resolution. By recording the laser emissions from neurons, we discovered that lasing emissions could be biologically modulated by intracellular activities and extracellular stimulation with >100-fold improvement in detection sensitivity over traditional fluorescence-based measurement. Finally, we showed that ultrasound can wirelessly activate neurons adsorbed with piezoelectric...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 8
Total Words: 6361
Unqiue Words: 2013

1.996 Mikeys
#6. Live-cell Imaging Analysis of Antimycin-Type Depsipeptides via Bioorthogonal Stimulated Raman Scattering Microscopy
Jeremy Allen Seidel, Yupeng Miao, William Porterfield, Wenlong Cai, Xuejun Zhu, Seong-Jong Kim, Fanghao Hu, Santi Bhattarai-Kline, Wei Min, Wenjun Zhang
Small-molecule natural products have been an essential source of pharmaceuticals to treat human diseases, but very little is known about their behavior inside dynamic, living human cells. Here, we demonstrate the first structure-activity-distribution study of complex natural products, the anti-cancer antimycin-type depsipeptides, using the emerging bioorthogonal Stimulated Raman Scattering (SRS) Microscopy. Our results show that the intracellular enrichment and distribution of these compounds are driven by their potency and specific protein targets, as well as the lipophilic nature of compounds.
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 10
Total Words: 3859
Unqiue Words: 1440

1.996 Mikeys
#7. Exploiting the unwanted: sulphate reduction enables phosphate recovery from energy-rich sludge during anaerobic digestion.
Celine Lippens, Jo De Vrieze
Anaerobic digestion is shifting from a single-purpose technology for renewable energy recovery from organic waste streams to a process for integrated resource recovery. The valorisation of high-rate energy- and phosphorus-rich sludge creates the opportunity for their combined recovery. This phosphate is present in a precipitated form in the sludge, and its release into the liquid phase is an important issue before recovery can be achieved. The objective of this research was to exploit the unwanted sulphate reduction process for the release of phosphate into the liquid phase during anaerobic digestion, thus, making it available for recovery. Two different treatments were considered, i.e., a control digester and a digester to which sulphate was added, each operated in triplicate for a period of 119 days. The control digester showed stable methane production at 628 ± 103 mL CH4 L-1 d-1, with a feedstock COD (chemical oxygen demand) conversion efficiency of 89.5 ± 14.6 %. In contrast, the digester with sulphate addition showed a 29.9...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 2
Total Words: 10648
Unqiue Words: 3535

1.992 Mikeys
#8. Numerical simulation for behind armor blunt trauma of human torso under non-penetrating ballistic impact
Fan Tang, Zerong Guo, Mengqi Yuan, Xinming Qian, Zhiming Du
A human torso finite element model with high bio-fidelity was developed to study the behind armor blunt trauma (BABT) of pistol cartridge on human torso with bulletproof composite structure (BCS) and the effect of buffer layer (expandable polyethylene, EPE) on BABT. The bulletproof structure was made of multilayered composite of aluminum alloy (AlSi10Mg) and thermoplastic polyurethanes (TPU), and the ANSYS/LS-DYNA software was used to simulate the blunt ballistic impact process of pistol cartridge on human torso. Results indicated that the BCS could resist the shooting speed of 515 m/s without being broken. During the process of pistol cartridge shooting the BCS, the energy of pistol cartridge was transmitted to the human organs through the BCS, thereby causing human injury. Moreover, the mechanical response parameters of various organs were determined by the distance between the human organs and the impact point. The sternal fracture and liver rupture were not produced based on the threshold stress of sternum and liver injury, no...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 5
Total Words: 7367
Unqiue Words: 1641

1.99 Mikeys
#9. Effect of motor learning on prosthetic finger control with an intuitive myoelectric decoder
Agamemnon Krasoulis, Sethu Vijayakumar, Kianoush Nazarpour
Machine learning-based myoelectric control is regarded as an intuitive paradigm, because of the mapping it creates between muscle co-activation patterns and prosthesis movements that aims to simulate the physiological pathways found in the human arm. Despite that, there has been evidence that closed-loop interaction with a classification-based interface results in user adaptation, which leads to performance improvement with experience. Recently, there has been a focus shift towards continuous prosthesis control, yet little is known about whether and how motor learning affects myoelectric control performance in dexterous, intuitive tasks. We investigate motor learning aspects of independent finger position control by conducting real-time experiments with 10 able-bodied and two transradial amputee subjects. We demonstrate that despite using an intuitive decoder, learning still occurs and leads to significant improvements in performance. We argue that this is due to the lack of an utterly natural control scheme, which is mainly...
more | pdf
Figures
Tweets
Github
None.
Youtube
None.
Other stats
Sample Sizes : None.
Authors: 3
Total Words: 10634
Unqiue Words: 2754

About

Assert is a website where the best academic papers on arXiv (computer science, math, physics), bioRxiv (biology), BITSS (reproducibility), EarthArXiv (earth science), engrXiv (engineering), LawArXiv (law), PsyArXiv (psychology), SocArXiv (social science), and SportRxiv (sport research) bubble to the top each day.

Papers are scored (in real-time) based on how verifiable they are (as determined by their Github repos) and how interesting they are (based on Twitter).

To see top papers, follow us on twitter @assertpub_ (arXiv), @assert_pub (bioRxiv), and @assertpub_dev (everything else).

To see beautiful figures extracted from papers, follow us on Instagram.

Tracking 99,586 papers.

Search
Sort results based on if they are interesting or reproducible.
Interesting
Reproducible
Feedback
Online
Stats
Tracking 99,586 papers.